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Anti-USP9X Rabbit Monoclonal Antibody #1135

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Flow cytometric analysis of USP9X expression in HepG2 cells using USP9X antibody (Cat#1135, 1:2,000). Green, isotype control; red, USP9X.
Immunocytochemical staining of HepG2 cells with USP9X antibody (Cat#1135, 1:1,000). Nuclei were stained blue with DAPI; USP9X was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-USP9X antibody (Cat#1135). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-USP9X antibody (Cat#1135, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    USP9X
  • 货号:
    1135
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-USP9X Rabbit Monoclonal Antibody
Aliases USP9X; Ubiquitin Specific Peptidase 9 X-Linked; DFFRX; MRX99; FAF; Ubiquitin Specific Protease 9, X Chromosome (Fat Facets-Like Drosophila); Probable Ubiquitin Carboxyl-Terminal Hydrolase FAF-X; Ubiquitin-Specific Protease 9, X Chromosome; Deubiquitinating Enzyme FAF-X; Ubiquitin Thioesterase FAF-X; Fat Facets In Mammals; HFAM; FAM; Ubiquitin Specific Peptidase 9, X-Linked (Fat Facets-Like, Drosophila); Ubiquitin Specific Protease 9, X-Linked (Fat Facets-Like, Drosophila); Ubiquitin-Specific Processing Protease FAF-X; Ubiquitin-Specific-Processing Protease FAF-X; Drosophila Fat Facets Related, X-Linked; Fat Facets Protein Related, X-Linked; Fat Facets Protein-Related, X-Linked; Ubiquitin Thiolesterase FAF-X; EC 3.4.19.12; EC 3.1.2.15; MRXS99F; XLID99; USP9
Background

Gene Name: USP9X

NCBI Gene Entry: 8239

UniProt Entry: Q93008

Application Information

Molecular Weight: Predicted, 292 kDa; observed, 292 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB2000

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human USP9x
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of USP9X expression in HepG2 cells using USP9X antibody (Cat#1135, 1:2,000). Green, isotype control; red, USP9X.
  • Immunocytochemical staining of HepG2 cells with USP9X antibody (Cat#1135, 1:1,000). Nuclei were stained blue with DAPI; USP9X was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
  • Western blotting analysis using anti-USP9X antibody (Cat#1135). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-USP9X antibody (Cat#1135, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of USP9X expression in HepG2 cells using USP9X antibody (Cat#1135, 1:2,000). Green, isotype control; red, USP9X.
Immunocytochemical staining of HepG2 cells with USP9X antibody (Cat#1135, 1:1,000). Nuclei were stained blue with DAPI; USP9X was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-USP9X antibody (Cat#1135). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-USP9X antibody (Cat#1135, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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