Anti-PP2A alpha + beta Rabbit Monoclonal Antibody - 产品中心

Anti-PP2A alpha + beta Rabbit Monoclonal Antibody #1228

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Flow cytometric analysis of PP2A alpha + beta expression in HAP-1 cells using PP2A alpha + beta antibody (Cat#1228, 1:2,000). Green, isotype control; red, PP2A alpha + beta.

Immunocytochemical staining of HepG2 cells with anti-PP2A alpha + beta antibody (Cat#1228, 1:1,000). Nuclei were stained blue with DAPI; PP2A alpha + beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-PP2A alpha + beta antibody (Cat#1228). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PP2A alpha + beta antibody (Cat#1228, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

PPP2CA/PPP2CB
货号：

1228
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-PP2A alpha + beta Rabbit Monoclonal Antibody
Aliases	PPP2CA; Protein Phosphatase 2 Catalytic Subunit Alpha; Serine/Threonine-Protein Phosphatase 2A Catalytic Subunit Alpha Isoform; Protein Phosphatase 2, Catalytic Subunit, Alpha Isozyme; Replication Protein C; EC 3.1.3.16; PP2A-Alpha; PP2Calpha; PP2AC; RP-C; Protein Phosphatase 2 (Formerly 2A), Catalytic Subunit, Alpha Isoform; Protein Phosphatase 2A Catalytic Subunit, Alpha Isoform; PP2CALPHA; NEDLBA; PP2Ac; PP2CA; HJS3
Background	Gene Name: PPP2CA/PPP2CB NCBI Gene Entry: 5515 UniProt Entry: P62714/P67775
Application Information	Molecular Weight: Predicted, 36 kDa; observed, 36 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB2300 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human alpha + beta PP2A
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of PP2A alpha + beta expression in HAP-1 cells using PP2A alpha + beta antibody (Cat#1228, 1:2,000). Green, isotype control; red, PP2A alpha + beta.
Immunocytochemical staining of HepG2 cells with anti-PP2A alpha + beta antibody (Cat#1228, 1:1,000). Nuclei were stained blue with DAPI; PP2A alpha + beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-PP2A alpha + beta antibody (Cat#1228). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PP2A alpha + beta antibody (Cat#1228, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

PPP2CA/PPP2CB
货号：

1228
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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