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Anti-CPSF3 Rabbit Monoclonal Antibody #1335

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Flow cytometric analysis of CPSF3 expression in HepG2 cells using anti-CPSF3 antibody (Cat#1335, 1:2,000). Green, isotype control; red, CPSF3.
Immunocytochemical staining of HepG2 cells with CPSF3 antibody (Cat#1335, 1:1,000). Nuclei were stained blue with DAPI; CPSF3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-CPSF3 antibody (Cat#1335). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CPSF3 antibody (Cat#1335, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    CPSF3
  • 货号:
    1335
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-CPSF3 Rabbit Monoclonal Antibody
Aliases CPSF3; Cleavage And Polyadenylation Specific Factor 3; CPSF73; CPSF-73; Cleavage And Polyadenylation Specificity Factor Subunit 3; Cleavage And Polyadenylation Specific Factor 3, 73kDa; MRNA 3'-End-Processing Endonuclease CPSF-73; YSH1; Cleavage And Polyadenylation Specificity Factor 73 KDa Subunit; Cleavage And Polyadenylation Specific Factor 3, 73kD Subunit; CPSF 73 KDa Subunit; EC 3.1.27.-; EC 3.1.27; NEDMHSN; NEDMHS
Background

Gene Name: CPSF3

NCBI Gene Entry: 51692

UniProt Entry: Q9UKF6

Application Information

Molecular Weight: Predicted, 77 kDa; observed, 77 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB3105

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human CPSF73
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Flow Cytometry (FCM) : 1:2,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of CPSF3 expression in HepG2 cells using anti-CPSF3 antibody (Cat#1335, 1:2,000). Green, isotype control; red, CPSF3.
  • Immunocytochemical staining of HepG2 cells with CPSF3 antibody (Cat#1335, 1:1,000). Nuclei were stained blue with DAPI; CPSF3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-CPSF3 antibody (Cat#1335). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CPSF3 antibody (Cat#1335, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of CPSF3 expression in HepG2 cells using anti-CPSF3 antibody (Cat#1335, 1:2,000). Green, isotype control; red, CPSF3.
Immunocytochemical staining of HepG2 cells with CPSF3 antibody (Cat#1335, 1:1,000). Nuclei were stained blue with DAPI; CPSF3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-CPSF3 antibody (Cat#1335). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CPSF3 antibody (Cat#1335, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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