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Anti-CD86 Rabbit Monoclonal Antibody #1355

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Flow cytometric analysis of CD86 expression in HT-1080 cells using CD86 antibody (Cat#1355, 1:2,000). Green, isotype control; red, CD86.
Immunocytochemical staining of HT-1080 cells with CD86 antibody (Cat#1355, 1:1,000). Nuclei were stained blue with DAPI; CD86 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Immunohistochemistry was performed on paraffin-embedded human tonsillitis using anti-CD86 antibody (Cat#1355, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Western blotting analysis using anti-CD86 antibody (Cat#1355). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD86 antibody (Cat#1355, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    CD86
  • 货号：
    1355
  • 应用：
    WB, FCM, IC, IHC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-CD86 Rabbit Monoclonal Antibody
Aliases CD86; CD86 Molecule; CD28LG2; B7.2; B7-2; CD86 Antigen (CD28 Antigen Ligand 2, B7-2 Antigen); T-Lymphocyte Activation Antigen CD86; CTLA-4 Counter-Receptor B7.2; FUN-1; BU63; B70; B-Lymphocyte Activation Antigen B7-2; B-Lymphocyte Antigen B7-2; Activation B7-2 Antigen; CD86 Antigen; LAB72
Background

Gene Name: CD86

NCBI Gene Entry: 942

UniProt Entry: P42081
Application Information

Molecular Weight: Predicted, 38 kDa; observed, 60-85 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB3385

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC), immunohistochemistry (IHC)
Immunogen A synthesized peptide derived from human CD86
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Flow Cytometry (FCM): 1:2,000
Immunohistochemistry (IHC): 1:100-1:200
Note This product is for research use only.
Picture
  • Flow cytometric analysis of CD86 expression in HT-1080 cells using CD86 antibody (Cat#1355, 1:2,000). Green, isotype control; red, CD86.
  • Immunocytochemical staining of HT-1080 cells with CD86 antibody (Cat#1355, 1:1,000). Nuclei were stained blue with DAPI; CD86 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Immunohistochemistry was performed on paraffin-embedded human tonsillitis using anti-CD86 antibody (Cat#1355, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Western blotting analysis using anti-CD86 antibody (Cat#1355). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD86 antibody (Cat#1355, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    CD86
  • 货号：
    1355
  • 应用：
    WB, FCM, IC, IHC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Flow cytometric analysis of CD86 expression in HT-1080 cells using CD86 antibody (Cat#1355, 1:2,000). Green, isotype control; red, CD86.
Immunocytochemical staining of HT-1080 cells with CD86 antibody (Cat#1355, 1:1,000). Nuclei were stained blue with DAPI; CD86 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Immunohistochemistry was performed on paraffin-embedded human tonsillitis using anti-CD86 antibody (Cat#1355, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Western blotting analysis using anti-CD86 antibody (Cat#1355). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD86 antibody (Cat#1355, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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