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Anti-Aquaporin 5 Rabbit Monoclonal Antibody #1370

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Flow cytometric analysis of Aquaporin 5 expression in C2C12 cells using Aquaporin 5 antibody (Cat#1370, 1:2,000). Green, isotype control; red, Aquaporin 5.
Immunocytochemical staining of C2C12 cells with Aquaporin 5 antibody (Cat#1370, 1:1,000). Nuclei were stained blue with DAPI; Aquaporin 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Aquaporin 5 antibody (Cat#1370). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Aquaporin 5 antibody (Cat#1370, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    AQP5
  • 货号:
    1370
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    M
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-Aquaporin 5 Rabbit Monoclonal Antibody
Aliases AQP5; Aquaporin 5; Aquaporin-5; AQP-5; PPKB
Background

Gene Name: AQP5

NCBI Gene Entry: 362

UniProt Entry: P55064

Application Information

Molecular Weight: Predicted, 28 kDa; observed, 24 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB3225

Species Reactivity: Mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human AQP5
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Aquaporin 5 expression in C2C12 cells using Aquaporin 5 antibody (Cat#1370, 1:2,000). Green, isotype control; red, Aquaporin 5.
  • Immunocytochemical staining of C2C12 cells with Aquaporin 5 antibody (Cat#1370, 1:1,000). Nuclei were stained blue with DAPI; Aquaporin 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
  • Western blotting analysis using anti-Aquaporin 5 antibody (Cat#1370). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Aquaporin 5 antibody (Cat#1370, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of Aquaporin 5 expression in C2C12 cells using Aquaporin 5 antibody (Cat#1370, 1:2,000). Green, isotype control; red, Aquaporin 5.
Immunocytochemical staining of C2C12 cells with Aquaporin 5 antibody (Cat#1370, 1:1,000). Nuclei were stained blue with DAPI; Aquaporin 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Aquaporin 5 antibody (Cat#1370). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Aquaporin 5 antibody (Cat#1370, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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