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Anti-Centromere protein C Rabbit Monoclonal Antibody #1430

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Flow cytometric analysis of Centromere protein C expression in C2C12 cells using Centromere protein C antibody (Cat#1430, 1:2,000). Green, isotype control; red, Centromere protein C.
Immunocytochemical staining of C2C12 cells with Centromere protein C antibody (Cat#1430, 1:1,000). Nuclei were stained blue with DAPI; Centromere protein C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Centromere protein C antibody (Cat#1430). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Centromere protein C antibody (Cat#1430, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    CENPC
  • 货号:
    1430
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-Centromere protein C Rabbit Monoclonal Antibody
Aliases CENPC; Centromere Protein C; CENP-C; Centromere Protein C 1; CENPC1; MIF2; Interphase Centromere Complex Protein 7; Centromere Autoantigen C; CENP-C 1; Hcp-4; Centromere Autoantigen C1; HCP-4; ICEN7
Background

Gene Name: CENPC

NCBI Gene Entry: 1060

UniProt Entry: Q03188

Application Information

Molecular Weight: Predicted, 107 kDa; observed, 107 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB3760

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human CENPC
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Centromere protein C expression in C2C12 cells using Centromere protein C antibody (Cat#1430, 1:2,000). Green, isotype control; red, Centromere protein C.
  • Immunocytochemical staining of C2C12 cells with Centromere protein C antibody (Cat#1430, 1:1,000). Nuclei were stained blue with DAPI; Centromere protein C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-Centromere protein C antibody (Cat#1430). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Centromere protein C antibody (Cat#1430, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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Flow cytometric analysis of Centromere protein C expression in C2C12 cells using Centromere protein C antibody (Cat#1430, 1:2,000). Green, isotype control; red, Centromere protein C.
Immunocytochemical staining of C2C12 cells with Centromere protein C antibody (Cat#1430, 1:1,000). Nuclei were stained blue with DAPI; Centromere protein C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Centromere protein C antibody (Cat#1430). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Centromere protein C antibody (Cat#1430, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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