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Anti-Caspase 5 Rabbit Monoclonal Antibody #1590

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Flow cytometric analysis of Caspase 5 expression in HT-1080 cells using Caspase 5 antibody (Cat#1590, 1:2,000). Green, isotype control; red, Caspase 5.

Immunocytochemical staining of HT-1080 cells with Caspase 5 antibody (Cat#1590, 1:1,000). Nuclei were stained blue with DAPI; Caspase 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Caspase 5 antibody (Cat#1590). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Caspase 5 antibody (Cat#1590, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

CASP5
货号：

1590
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-Caspase 5 Rabbit Monoclonal Antibody
Aliases	CASP5; Caspase 5; Caspase 5, Apoptosis-Related Cysteine Peptidase; Caspase 5, Apoptosis-Related Cysteine Protease; Protease ICH-3; ICE(Rel)III; Protease TY; Caspase-5; ICE(Rel)-III; EC 3.4.22.58; TY Protease; ICE(REL)III; ICEREL-III; EC 3.4.22; CASP-5; ICH-3; ICH3
Background	Gene Name: CASP5 NCBI Gene Entry: 838 UniProt Entry: P51878
Application Information	Molecular Weight: Predicted, 50 kDa; observed, 47 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB3315 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Caspase 5
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Caspase 5 expression in HT-1080 cells using Caspase 5 antibody (Cat#1590, 1:2,000). Green, isotype control; red, Caspase 5.
Immunocytochemical staining of HT-1080 cells with Caspase 5 antibody (Cat#1590, 1:1,000). Nuclei were stained blue with DAPI; Caspase 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Caspase 5 antibody (Cat#1590). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Caspase 5 antibody (Cat#1590, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

CASP5
货号：

1590
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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