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Anti-CDC34 Rabbit Monoclonal Antibody #1595

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Flow cytometric analysis of CDC34 expression in HT-1080 cells using CDC34 antibody (Cat#1595, 1:2,000). Green, isotype control; red, CDC34.

Immunocytochemical staining of HT-1080 cells with CDC34 antibody (Cat#1595, 1:1,000). Nuclei were stained blue with DAPI; CDC34 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-CDC34 antibody (Cat#1595). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CDC34 antibody (Cat#1595, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

CDC34
货号：

1595
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-CDC34 Rabbit Monoclonal Antibody
Aliases	CDC34; Cell Division Cycle 34, Ubiqiutin Conjugating Enzyme; UBE2R1; E2-CDC34; UBC3; (E3-Independent) E2 Ubiquitin-Conjugating Enzyme R1; Ubiquitin-Conjugating Enzyme E2-CDC34; Ubiquitin-Conjugating Enzyme E2 R1; E2 Ubiquitin-Conjugating Enzyme R1; Ubiquitin-Protein Ligase R1; UBCH3; Ubiquitin-Conjugating Enzyme E2-32 KDA Complementing; Ubiquitin-Conjugating Enzyme E2-32 KDa Complementing; Ubiquitin Conjugating Enzyme E2 R1; Cell Division Cycle 34 Homolog; Ubiquitin Carrier Protein; EC 2.3.2.23; EC 2.3.2.24; EC 6.3.2.19
Background	Gene Name: CDC34 NCBI Gene Entry: 997 UniProt Entry: P49427
Application Information	Molecular Weight: Predicted, 27 kDa; observed, 27 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB3435 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human CDC34
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of CDC34 expression in HT-1080 cells using CDC34 antibody (Cat#1595, 1:2,000). Green, isotype control; red, CDC34.
Immunocytochemical staining of HT-1080 cells with CDC34 antibody (Cat#1595, 1:1,000). Nuclei were stained blue with DAPI; CDC34 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-CDC34 antibody (Cat#1595). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CDC34 antibody (Cat#1595, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

CDC34
货号：

1595
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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