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Anti-Aconitase 1 Rabbit Monoclonal Antibody #1648

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Flow cytometric analysis of Aconitase 1 expression in C2C12 cells using Aconitase 1 antibody (Cat#1648, 1:2000). Green, isotype control; red, Aconitase 1
Immunocytochemical staining of C2C12 cells with Aconitase 1 antibody (Cat#1648, 1:1,000). Nuclei were stained blue with DAPI; Aconitase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Aconitase 1 antibody (Cat#1648). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Aconitase 1 antibody (Cat#1648, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    ACO1
  • 货号：
    1648
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-Aconitase 1 Rabbit Monoclonal Antibody
Aliases ACO1; Aconitase 1; IRP1; IREBP; IREB1; Iron-Responsive Element-Binding Protein 1; Aconitate Hydratase, Cytoplasmic; Cytoplasmic Aconitate Hydratase; Ferritin Repressor Protein; Iron Regulatory Protein 1; Cytosplasmic Aconitase; Aconitase 1, Soluble; Citrate Hydro-Lyase; Soluble Aconitase; EC 4.2.1.3; Epididymis Secretory Sperm Binding Protein; Epididymis Luminal Protein 60; Cytoplasmic Aconitase; Aconitase; IRE-BP 1; EC 4.2.1; IREBP1; ACONS; HEL60
Background

Gene Name: ACO1

NCBI Gene Entry: 48

UniProt Entry: P21399
Application Information

Molecular Weight: Predicted, 98 kDa; observed, 95,130 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB3915

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Aconitase 1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Aconitase 1 expression in C2C12 cells using Aconitase 1 antibody (Cat#1648, 1:2000). Green, isotype control; red, Aconitase 1
  • Immunocytochemical staining of C2C12 cells with Aconitase 1 antibody (Cat#1648, 1:1,000). Nuclei were stained blue with DAPI; Aconitase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
  • Western blotting analysis using anti-Aconitase 1 antibody (Cat#1648). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Aconitase 1 antibody (Cat#1648, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    ACO1
  • 货号：
    1648
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Flow cytometric analysis of Aconitase 1 expression in C2C12 cells using Aconitase 1 antibody (Cat#1648, 1:2000). Green, isotype control; red, Aconitase 1
Immunocytochemical staining of C2C12 cells with Aconitase 1 antibody (Cat#1648, 1:1,000). Nuclei were stained blue with DAPI; Aconitase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Aconitase 1 antibody (Cat#1648). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Aconitase 1 antibody (Cat#1648, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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