Anti-Phospho-B Raf (T401) Rabbit Monoclonal Antibody - 产品中心

Anti-Phospho-B Raf (T401) Rabbit Monoclonal Antibody #1658

一键复制产品信息

返回列表

Flow cytometric analysis of Phospho-B Raf (T401) expression in H9c2 cells using Phospho-B Raf (T401) antibody (Cat#1658, 1:2,000). Green, isotype control; red, Phospho-B Raf (T401).

Immunocytochemical staining of H9C2 cells with Phospho-B Raf (T401) antibody (Cat#1658, 1:1,000). Nuclei were stained blue with DAPI; Phospho-B Raf (T401) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Phospho-B Raf (T401) antibody (Cat#1658). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-B Raf (T401) antibody (Cat#1658, 1:5,000) and HRP-conjugated goat anti -rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

BRAF
货号：

1658
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-Phospho-B Raf (T401) Rabbit Monoclonal Antibody
Aliases	BRAF; B-Raf Proto-Oncogene, Serine/Threonine Kinase; BRAF1; BRAF-1; V-Raf Murine Sarcoma Viral Oncogene Homolog B1; V-Raf Murine Sarcoma Viral Oncogene Homolog B; Serine/Threonine-Protein Kinase B-Raf; Proto-Oncogene B-Raf; RAFB1; B-Raf Proto-Oncogene Serine/Threonine-Protein Kinase (P94); Murine Sarcoma Viral (V-Raf) Oncogene Homolog B1; B-Raf Serine/Threonine-Protein; 94 KDa B-Raf Protein; EC 2.7.11.1; B-RAF1; B-Raf; NS7; P94
Background	Gene Name: BRAF NCBI Gene Entry: 673 UniProt Entry: P15056
Application Information	Molecular Weight: Predicted, 84 kDa; observed, 85 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB3960 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Phospho-B Raf (T401)
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Phospho-B Raf (T401) expression in H9c2 cells using Phospho-B Raf (T401) antibody (Cat#1658, 1:2,000). Green, isotype control; red, Phospho-B Raf (T401).
Immunocytochemical staining of H9C2 cells with Phospho-B Raf (T401) antibody (Cat#1658, 1:1,000). Nuclei were stained blue with DAPI; Phospho-B Raf (T401) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Phospho-B Raf (T401) antibody (Cat#1658). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-B Raf (T401) antibody (Cat#1658, 1:5,000) and HRP-conjugated goat anti -rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

BRAF
货号：

1658
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

首页

关于我们

善本标准

产品中心

定制服务

信号通路

促销活动

技术支持

新闻资讯

首页