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Anti-ATOH1 Rabbit Monoclonal Antibody #1756

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Immunocytochemical staining of Hela cells with ATOH1 antibody (Cat#1756, 1:1,000). Nuclei were stained blue with DAPI; ATOH1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-ATOH1 antibody (Cat#1756). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATOH1 antibody (Cat#1756, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    ATOH1
  • 货号:
    1756
  • 应用:
    WB, IC
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-ATOH1 Rabbit Monoclonal Antibody
Aliases ATOH1; Atonal BHLH Transcription Factor 1; HATH1; BHLHa14; MATH-1; Atonal Homolog BHLH Transcription Factor 1; Class A Basic Helix-Loop-Helix Protein 14; Basic Helix-Loop-Helix Family Member A14; Helix-Loop-Helix Protein HATH-1; Transcription Factor ATOH1; Protein Atonal Homolog 1; BHLHA14; ATH1; Atonal Homolog 1 (Drosophila); Atonal Homolog 1; DFNA89; Math1; MATH1
Background

Gene Name: ATOH1

NCBI Gene Entry: 474

UniProt Entry: Q92858

Application Information

Molecular Weight: Predicted, 38 kDa; observed, 38 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB4335

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human MATH1/HATH1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Immunocytochemical staining of Hela cells with ATOH1 antibody (Cat#1756, 1:1,000). Nuclei were stained blue with DAPI; ATOH1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-ATOH1 antibody (Cat#1756). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATOH1 antibody (Cat#1756, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Immunocytochemical staining of Hela cells with ATOH1 antibody (Cat#1756, 1:1,000). Nuclei were stained blue with DAPI; ATOH1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-ATOH1 antibody (Cat#1756). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATOH1 antibody (Cat#1756, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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