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Anti-Nesprin3 Rabbit Monoclonal Antibody #1830

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Immunocytochemical staining of HeLa cells with Nesprin3 antibody (Cat#1830, 1:1,000). Nuclei were stained blue with DAPI; Nesprin3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Nesprin3 antibody (Cat#1830). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Nesprin3 antibody (Cat#1830, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    SYNE3
  • 货号：
    1830
  • 应用：
    WB, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-Nesprin3 Rabbit Monoclonal Antibody
Aliases SYNE3; Spectrin Repeat Containing Nuclear Envelope Family Member 3; Nesprin-3; LINC00341; NET53; Long Intergenic Non-Protein Coding RNA 341; Nuclear Envelope Spectrin Repeat Protein 3; KASH Domain-Containing Protein 3; NCRNA00341; C14orf139; FLJ25605; C14orf49; Nesp3; KASH3; Chromosome 14 Open Reading Frame 139; Chromosome 14 Open Reading Frame 49; Uncharacterized Protein C14orf113; Non-Protein Coding RNA 341; C14ORF139; NESPRIN-3; C14ORF49; NESP3
Background

Gene Name: SYNE3

NCBI Gene Entry: 161176

UniProt Entry: Q6ZMZ3
Application Information

Molecular Weight: Predicted, 112 kDa; observed, 100 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB4665

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Nesprin3
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Immunocytochemical staining of HeLa cells with Nesprin3 antibody (Cat#1830, 1:1,000). Nuclei were stained blue with DAPI; Nesprin3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-Nesprin3 antibody (Cat#1830). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Nesprin3 antibody (Cat#1830, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    SYNE3
  • 货号：
    1830
  • 应用：
    WB, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Immunocytochemical staining of HeLa cells with Nesprin3 antibody (Cat#1830, 1:1,000). Nuclei were stained blue with DAPI; Nesprin3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Nesprin3 antibody (Cat#1830). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Nesprin3 antibody (Cat#1830, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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