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Anti-TEAD1/2/3/4 Rabbit Monoclonal Antibody #1833

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Flow cytometric analysis of TEAD1/2/3/4 expression in HepG2 cells using TEAD1/2/3/4 antibody (Cat#1833, 1:2,000). Green, isotype control; red, TEAD1/2/3/4.
Immunocytochemical staining of HepG2 cells with TEAD1/2/3/4 antibody (Cat#1833, 1:1,000). Nuclei were stained blue with DAPI; TEAD1/2/3/4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-TEAD1/2/3/4 antibody (Cat#1833). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TEAD1/2/3/4 antibody (Cat#1833, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    TEAD1/2/3/4
  • 货号:
    1833
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-TEAD1/2/3/4 Rabbit Monoclonal Antibody
Aliases TEAD1; TEA Domain Transcription Factor 1; TEF-1; TCF13; TEA Domain Family Member 1 (SV40 Transcriptional Enhancer Factor); Transcriptional Enhancer Factor TEF-1; Transcriptional Enhancer Factor 1; Transcription Factor 13; Protein GT-IIC; NTEF-1; TCF-13; TEAD-1; AA; Atrophia Areata, Peripapillary Chorioretinal Degeneration; TEA Domain Family Member 1; REF1; TEF1; TEAD4; TEA Domain Transcription Factor 4; TEA Domain Family Member 4; TCF13L1; TEFR-1; EFTR-2; TEF-3; Transcriptional Enhancer Factor TEF-3; Transcriptional Enhancer Factor 3; Transcription Factor 13-Like 1; Transcription Factor RTEF-1; RTEF-1; RTEF1; TEF3; Transcriptional Enhancer Factor 1-Related; Related Transcription Enhancer Factor 1B; HRTEF-1B; TEAD-4; TEAD2; TEA Domain Transcription Factor 2; TEF4; TEA Domain Family Member 2; TEF-4; ETF; Transcriptional Enhancer Factor TEF-4; TEAD-2; TEAD3; TEA Domain Transcription Factor 3; TEA Domain Family Member 3; ETFR-1; TEF-5; TEAD5; Transcriptional Enhancer Factor TEF-5; DTEF-1; TEAD-3; TEF5; Transcriptional Enhancer Factor TEF-5 (DTEF-1); Transcriptional Enhancer Factor 5; TEA Domain Family Member 5
Background

Gene Name: TEAD1/2/3/4

NCBI Gene Entry: 7003/7004/8463/7005

UniProt Entry: P28347/Q15561/Q15562/Q99594

Application Information

Molecular Weight: Predicted, 48 kDa; observed, 54 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB4680

Species Reactivity: Human, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human TEAD1 + TEAD2 + TEAD3 + TEAD4
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of TEAD1/2/3/4 expression in HepG2 cells using TEAD1/2/3/4 antibody (Cat#1833, 1:2,000). Green, isotype control; red, TEAD1/2/3/4.
  • Immunocytochemical staining of HepG2 cells with TEAD1/2/3/4 antibody (Cat#1833, 1:1,000). Nuclei were stained blue with DAPI; TEAD1/2/3/4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-TEAD1/2/3/4 antibody (Cat#1833). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TEAD1/2/3/4 antibody (Cat#1833, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of TEAD1/2/3/4 expression in HepG2 cells using TEAD1/2/3/4 antibody (Cat#1833, 1:2,000). Green, isotype control; red, TEAD1/2/3/4.
Immunocytochemical staining of HepG2 cells with TEAD1/2/3/4 antibody (Cat#1833, 1:1,000). Nuclei were stained blue with DAPI; TEAD1/2/3/4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-TEAD1/2/3/4 antibody (Cat#1833). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TEAD1/2/3/4 antibody (Cat#1833, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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