Anti-Splicing factor 3a subunit 3 Rabbit Monoclonal Antibody - 产品中心

Anti-Splicing factor 3a subunit 3 Rabbit Monoclonal Antibody #1867

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Flow cytometric analysis of Splicing factor 3a subunit 3 expression in HeLa cells using Splicing factor 3a subunit 3 antibody (Cat#1867, 1:2,000). Green, isotype control; red, Splicing factor 3a subunit 3.

Immunocytochemical staining of Hela cells with anti-Splicing factor 3a subunit 3 antibody (Cat#1867,1:1000) . Nuclei were stained blue with DAPI; Splicing factor 3a subunit 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.

Western blotting analysis using anti-Splicing factor 3a subunit 3 antibody (Cat#1867). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Splicing factor 3a subunit 3 antibody (Cat#1867, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

SF3A3
货号：

1867
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-Splicing factor 3a subunit 3 Rabbit Monoclonal Antibody
Aliases	SF3A3; Splicing Factor 3a Subunit 3; SAP61; SF3a60; PRPF9; PRP9; Splicing Factor 3a, Subunit 3, 60kDa; Splicing Factor 3a, Subunit 3, 60kD; Splicing Factor 3A Subunit 3; SAP 61; Pre-MRNA Splicing Factor SF3a (60kD); Spliceosome Associated Protein 61; Spliceosome-Associated Protein 61; SF3A60
Background	Gene Name: SF3A3 NCBI Gene Entry: 10946 UniProt Entry: Q12874
Application Information	Molecular Weight: Predicted, 59 kDa; observed, 59 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB4775 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human SF3A3
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000 Flow Cytometry (FCM): 1:2,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Splicing factor 3a subunit 3 expression in HeLa cells using Splicing factor 3a subunit 3 antibody (Cat#1867, 1:2,000). Green, isotype control; red, Splicing factor 3a subunit 3.
Immunocytochemical staining of Hela cells with anti-Splicing factor 3a subunit 3 antibody (Cat#1867,1:1000) . Nuclei were stained blue with DAPI; Splicing factor 3a subunit 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-Splicing factor 3a subunit 3 antibody (Cat#1867). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Splicing factor 3a subunit 3 antibody (Cat#1867, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

SF3A3
货号：

1867
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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