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Anti-Docking protein 1 Rabbit Monoclonal Antibody #1933

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Flow cytometric analysis of Docking protein 1 expression in C2C12 cells using Docking protein 1 antibody (Cat#1933, 1:2,000). Green, isotype control; red, Docking protein 1.

Immunocytochemical staining of HepG2 cells with anti-Docking protein 1 antibody (Cat#1933, 1:1,000) . Nuclei were stained blue with anti-DAPI; Docking protein 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-Docking protein 1 antibody (Cat#1933). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Docking protein 1 antibody (Cat#1933, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

DOK1
货号：

1933
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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Information

Product Name	Anti-Docking protein 1 Rabbit Monoclonal Antibody
Aliases	DOK1; Docking Protein 1; Downstream Of Tyrosine Kinase 1; P62DOK; Pp62; Docking Protein 1, 62kDa (Downstream Of Tyrosine Kinase 1); Docking Protein 1, 62kD (Downstream Of Tyrosine Kinase 1); P62(Dok); P62dok
Background	Gene Name: DOK1 NCBI Gene Entry: 1796 UniProt Entry: Q99704
Application Information	Molecular Weight: Predicted, 52 kDa; observed, 65 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB5185 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human DOK1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000 Flow Cytometry (FCM): 1:2,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Docking protein 1 expression in C2C12 cells using Docking protein 1 antibody (Cat#1933, 1:2,000). Green, isotype control; red, Docking protein 1.
Immunocytochemical staining of HepG2 cells with anti-Docking protein 1 antibody (Cat#1933, 1:1,000) . Nuclei were stained blue with anti-DAPI; Docking protein 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-Docking protein 1 antibody (Cat#1933). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Docking protein 1 antibody (Cat#1933, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

DOK1
货号：

1933
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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