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Anti-eIF1A Rabbit Monoclonal Antibody #1955

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Flow cytometric analysis of eIF1A expression in HeLa cells using eIF1A antibody (Cat#1955, 1:2,000). Green, isotype control; red, eIF1A.

Immunocytochemical staining of Hela cells with anti-EIF1AX antibody (Cat#1955, 1:1,000). Nuclei were stained blue with DAPI; EIF1AX was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-eIF1A antibody (Cat#1955). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-eIF1A antibody (Cat#1955, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

EIF1AX
货号：

1955
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-eIF1A Rabbit Monoclonal Antibody
Aliases	EIF1AX; Eukaryotic Translation Initiation Factor 1A X-Linked; EIF-4C; EIF-1A; EIF1A; EIF4C; Eukaryotic Translation Initiation Factor 1A, X-Chromosomal; Eukaryotic Translation Initiation Factor 1A, X Chromosome; Eukaryotic Translation Initiation Factor 4C; EIF1A X Isoform; Eukaryotic Translation Initiation Factor 1A, X-Linked; Putative Eukaryotic Translation Initiation Factor 1A; EIF-1A X Isoform; EIF1AP1
Background	Gene Name: EIF1AX NCBI Gene Entry: 1964 UniProt Entry: P47813
Application Information	Molecular Weight: Predicted, 16 kDa; observed, 18 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB5290 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human eIF1A
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000 Flow Cytometry (FCM): 1:2,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of eIF1A expression in HeLa cells using eIF1A antibody (Cat#1955, 1:2,000). Green, isotype control; red, eIF1A.
Immunocytochemical staining of Hela cells with anti-EIF1AX antibody (Cat#1955, 1:1,000). Nuclei were stained blue with DAPI; EIF1AX was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-eIF1A antibody (Cat#1955). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-eIF1A antibody (Cat#1955, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

EIF1AX
货号：

1955
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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