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Anti-Cytochrome P450 17A1/CYP17A1 Rabbit Monoclonal Antibody #1961

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Flow cytometric analysis of Cytochrome P450 17A1/CYP17A1 expression in HeLa cells using Cytochrome P450 17A1/CYP17A1 antibody (Cat#1961, 1:2,000). Green, isotype control; red, Cytochrome P450 17A1/CYP17A1.
Immunocytochemical staining of HepG2 cells with anti-cytochrome P450 17A1/CYP17A1 antibody (Cat#1961, 1:1,000). Nuclei were stained blue with DAPI; Cytochrome P450 17A1/CYP17A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Cytochrome P450 17A1/CYP17A1 antibody (Cat#1961). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Cytochrome P450 17A1/CYP17A1 antibody (Cat#1961, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    CYP17A1
  • 货号:
    1961
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-Cytochrome P450 17A1/CYP17A1 Rabbit Monoclonal Antibody
Aliases CYP17A1; Cytochrome P450 Family 17 Subfamily A Member 1; S17AH 2 3 4 5; Steroid 17-Alpha; Monooxygenase; P450C17; CYP17; CPT7; Cytochrome P450, Subfamily XVII (Steroid 17-Alpha-Hydroxylase), Adrenal Hyperplasia; Cytochrome P450, Family 17, Subfamily A, Polypeptide 1; Steroid 17-Alpha-Hydroxylase/17,20 Lyase; 17-Alpha-Hydroxyprogesterone Aldolase; Cytochrome P450 17A1; Cytochrome P450-C17; Cytochrome P450c17; CYPXVII; Cytochrome P450 XVIIA1; EC 1.14.14.19; EC 1.14.14.32; EC 1.14.99.9; EC 1.14.99
Background

Gene Name: CYP17A1

NCBI Gene Entry: 1586

UniProt Entry: P05093

Application Information

Molecular Weight: Predicted, 57 kDa; observed, 50,120 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB1910

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human Cytochrome P450 17A1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Cytochrome P450 17A1/CYP17A1 expression in HeLa cells using Cytochrome P450 17A1/CYP17A1 antibody (Cat#1961, 1:2,000). Green, isotype control; red, Cytochrome P450 17A1/CYP17A1.
  • Immunocytochemical staining of HepG2 cells with anti-cytochrome P450 17A1/CYP17A1 antibody (Cat#1961, 1:1,000). Nuclei were stained blue with DAPI; Cytochrome P450 17A1/CYP17A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-Cytochrome P450 17A1/CYP17A1 antibody (Cat#1961). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Cytochrome P450 17A1/CYP17A1 antibody (Cat#1961, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of Cytochrome P450 17A1/CYP17A1 expression in HeLa cells using Cytochrome P450 17A1/CYP17A1 antibody (Cat#1961, 1:2,000). Green, isotype control; red, Cytochrome P450 17A1/CYP17A1.
Immunocytochemical staining of HepG2 cells with anti-cytochrome P450 17A1/CYP17A1 antibody (Cat#1961, 1:1,000). Nuclei were stained blue with DAPI; Cytochrome P450 17A1/CYP17A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Cytochrome P450 17A1/CYP17A1 antibody (Cat#1961). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Cytochrome P450 17A1/CYP17A1 antibody (Cat#1961, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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