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Anti-FHIT Rabbit Monoclonal Antibody #1983

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Flow cytometric analysis of FHIT expression in HeLa cells using FHIT antibody (Cat#1983, 1:2,000). Green, isotype control; red, FHIT.
Immunocytochemical staining of Hela cells with anti-FHIT antibody (Cat #1983, 1:1,000) . Nuclei were stained blue with DAPI; FHIT was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-FHIT antibody (Cat#1983). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FHIT antibody (Cat#1983, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    FHIT
  • 货号:
    1983
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-FHIT Rabbit Monoclonal Antibody
Aliases FHIT; Fragile Histidine Triad Diadenosine Triphosphatase; Bis(5'-Adenosyl)-Triphosphatase; AP3Aase; FRA3B; Diadenosine 5',5'''-P1,P3-Triphosphate Hydrolase; Adenylylsulfate-Ammonia Adenylyltransferase; Adenosine 5'-Monophosphoramidase FHIT; Dinucleosidetriphosphatase; Adenylylsulfatase; AP3A Hydrolase; Fragile Histidine Triad Protein; Fragile Histidine Triad Gene; Fragile Histidine Triad; EC 3.6.1.29; EC 2.7.7.51; EC 3.9.1.-; EC 3.6.2.1; AP3AASE
Background

Gene Name: FHIT

NCBI Gene Entry: 2272

UniProt Entry: P49789

Application Information

Molecular Weight: Predicted, 17 kDa; observed, 17 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB5415

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human FHIT
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of FHIT expression in HeLa cells using FHIT antibody (Cat#1983, 1:2,000). Green, isotype control; red, FHIT.
  • Immunocytochemical staining of Hela cells with anti-FHIT antibody (Cat #1983, 1:1,000) . Nuclei were stained blue with DAPI; FHIT was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
  • Western blotting analysis using anti-FHIT antibody (Cat#1983). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FHIT antibody (Cat#1983, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of FHIT expression in HeLa cells using FHIT antibody (Cat#1983, 1:2,000). Green, isotype control; red, FHIT.
Immunocytochemical staining of Hela cells with anti-FHIT antibody (Cat #1983, 1:1,000) . Nuclei were stained blue with DAPI; FHIT was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-FHIT antibody (Cat#1983). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FHIT antibody (Cat#1983, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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