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Anti-Glutamate decarboxylase 1 Rabbit Monoclonal Antibody #2002

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Flow cytometric analysis of Glutamate decarboxylase 1 expression in HepG2 cells using Glutamate decarboxylase 1 antibody (Cat#2002, 1:2,000). Green, isotype control; red, Glutamate decarboxylase 1.

Immunocytochemical staining of HepG2 cells with anti-Glutamate decarboxylase 1 antibody (Cat#2002, 1:1,000). Nuclei were stained blue with DAPI; Glutamate decarboxylase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Glutamate decarboxylase 1 antibody (Cat#2002). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Glutamate decarboxylase 1 antibody (Cat#2002, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

GAD1
货号：

2002
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-Glutamate decarboxylase 1 Rabbit Monoclonal Antibody
Aliases	GAD1; Glutamate Decarboxylase 1; GAD; Glutamate Decarboxylase 1 (Brain, 67kDa); 67 KDa Glutamic Acid Decarboxylase; EC 4.1.1.15; GAD-67; Glutamate Decarboxylase 1 (Brain, 67kD); Glutamate Decarboxylase 67 KDa Isoform; EC 4.1.; CPSQ1; DEE89; GAD67; SCP
Background	Gene Name: GAD1 NCBI Gene Entry: 2571 UniProt Entry: Q99259
Application Information	Molecular Weight: Predicted, 67 kDa; observed, 67 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB5510 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen	A synthesized peptide derived from human GAD67
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000 Flow Cytometry (FCM): 1:2,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Glutamate decarboxylase 1 expression in HepG2 cells using Glutamate decarboxylase 1 antibody (Cat#2002, 1:2,000). Green, isotype control; red, Glutamate decarboxylase 1.
Immunocytochemical staining of HepG2 cells with anti-Glutamate decarboxylase 1 antibody (Cat#2002, 1:1,000). Nuclei were stained blue with DAPI; Glutamate decarboxylase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Glutamate decarboxylase 1 antibody (Cat#2002). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Glutamate decarboxylase 1 antibody (Cat#2002, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

GAD1
货号：

2002
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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