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Anti-C4 Rabbit Monoclonal Antibody #2038

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Flow cytometric analysis of C4 expression in C2C12 cells using C4 antibody (2038, 1:2,000). Green, isotype control; red, C4.
Immunocytochemical staining of HepG2 cells with anti-C4 antibody (Cat#2038, 1:1,000) . Nuclei were stained blue with anti-DAPI; C4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-C4 antibody (Cat#2038). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-C4 antibody (Cat#2038, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    C4A
  • 货号:
    2038
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    M
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-C4 Rabbit Monoclonal Antibody
Aliases C4A; Complement C4A (Rodgers Blood Group); CPAMD2; CO4; C4A3; C4A2; C4A4; C4A6; C4S; C4; RG; C3 And PZP-Like Alpha-2-Macroglobulin Domain-Containing Protein; Complement Component 4A (Rodgers Blood Group); Acidic Complement C4; Complement C4-A; C4B; MHC Class III Region Complement; Complement Component 4A; Rodgers Form Of C4; C4A Anaphylatoxin; EC 2.1.1.144; Acidic C4; EC 2.7.11; C4AD
Background

Gene Name: C4A

NCBI Gene Entry: 720

UniProt Entry: P0C0L4

Application Information

Molecular Weight: Predicted, 193 kDa; observed, 210 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB4985

Species Reactivity: Mouse

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human C4
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of C4 expression in C2C12 cells using C4 antibody (2038, 1:2,000). Green, isotype control; red, C4.
  • Immunocytochemical staining of HepG2 cells with anti-C4 antibody (Cat#2038, 1:1,000) . Nuclei were stained blue with anti-DAPI; C4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-C4 antibody (Cat#2038). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-C4 antibody (Cat#2038, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of C4 expression in C2C12 cells using C4 antibody (2038, 1:2,000). Green, isotype control; red, C4.
Immunocytochemical staining of HepG2 cells with anti-C4 antibody (Cat#2038, 1:1,000) . Nuclei were stained blue with anti-DAPI; C4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-C4 antibody (Cat#2038). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-C4 antibody (Cat#2038, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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