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Anti-Fibrillarin Rabbit Monoclonal Antibody #2047

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Flow cytometric analysis of Fibrillarin expression in HepG2 cells using Fibrillarin antibody (Cat#2047, 1:2,000). Green, isotype control; red, Fibrillarin.

Immunocytochemical staining of HepG2 cells with anti-Fibrillarin antibody (Cat#2047, 1:1000) . Nuclei were stained blue with DAPI; Fibrillarin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-Fibrillarin antibody (Cat#2047). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Fibrillarin antibody (Cat#2047, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

FBL
货号：

2047
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-Fibrillarin Rabbit Monoclonal Antibody
Aliases	FBL; Fibrillarin; FLRN; RNU3IP1; FIB; U6 SnRNA 2'-O-Methyltransferase Fibrillarin; RRNA 2'-O-Methyltransferase Fibrillarin; 34 KDa Nucleolar Scleroderma Antigen; Histone-Glutamine Methyltransferase; Nop1; RNA, U3 Small Nucleolar Interacting Protein 1; 34-KD Nucleolar Scleroderma Antigen; EC 2.1.1.37; EC 2.1.1.-; EC 2.1.; NOP1; FIB1
Background	Gene Name: FBL NCBI Gene Entry: 2091 UniProt Entry: P22087
Application Information	Molecular Weight: Predicted, 34 kDa; observed, 33 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB5735 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Fibrillarin
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Fibrillarin expression in HepG2 cells using Fibrillarin antibody (Cat#2047, 1:2,000). Green, isotype control; red, Fibrillarin.
Immunocytochemical staining of HepG2 cells with anti-Fibrillarin antibody (Cat#2047, 1:1000) . Nuclei were stained blue with DAPI; Fibrillarin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-Fibrillarin antibody (Cat#2047). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Fibrillarin antibody (Cat#2047, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

FBL
货号：

2047
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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