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Anti-GBA Rabbit Monoclonal Antibody #2055

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Flow cytometric analysis of GBA expression in C2C12 cells using GBA antibody (Cat#2055, 1:2,000). Green, isotype control; red, GBA.
Immunocytochemical staining of HepG2 cells with anti-GBA antibody (Cat#2055, 1:1,000) . Nuclei were stained blue with anti-DAPI; GBA was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-GBA antibody (Cat#2055). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GBA antibody (Cat#2055, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    GBA1
  • 货号:
    2055
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-GBA Rabbit Monoclonal Antibody
Aliases GBA1; Glucosylceramidase Beta 1GLUC; GBA; D-Glucosyl-N-Acylsphingosine Glucohydrolase; Lysosomal Cholesterol Glycosyltransferase; Lysosomal Acid Glucosylceramidase; Cholesterol Glucosyltransferase; Lysosomal Galactosylceramidase; Cholesteryl-Beta-Glucosidase; Lysosomal Glycosylceramidase; Beta-Glucosylceramidase; Glucosidase, Beta, Acid; Beta-Glucocerebrosidase; Acid Beta-Glucosidase; Lysosomal Acid GCase; Glucocerebrosidase; Imiglucerase; Alglucerase; EC 3.2.1.45; Beta-GC; SGTase; Glucosidase, Beta; Acid (Includes Glucosylceramidase); Glucosylceramidase-Like Protein; Lysosomal Glucocerebrosidase; Glucosylceramidase Beta; Glucosylceramidase; EC 3.2.1.46; EC 2.4.1.-; EC 3.2.1.-; GCB; GC
Background

Gene Name: GBA1

NCBI Gene Entry: 2629

UniProt Entry: P04062

Application Information

Molecular Weight: Predicted, 60 kDa; observed, 70 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB5775

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human GBA
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of GBA expression in C2C12 cells using GBA antibody (Cat#2055, 1:2,000). Green, isotype control; red, GBA.
  • Immunocytochemical staining of HepG2 cells with anti-GBA antibody (Cat#2055, 1:1,000) . Nuclei were stained blue with anti-DAPI; GBA was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
  • Western blotting analysis using anti-GBA antibody (Cat#2055). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GBA antibody (Cat#2055, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of GBA expression in C2C12 cells using GBA antibody (Cat#2055, 1:2,000). Green, isotype control; red, GBA.
Immunocytochemical staining of HepG2 cells with anti-GBA antibody (Cat#2055, 1:1,000) . Nuclei were stained blue with anti-DAPI; GBA was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-GBA antibody (Cat#2055). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GBA antibody (Cat#2055, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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