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Anti-CYP2D6 Rabbit Monoclonal Antibody #2060

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Flow cytometric analysis of CYP2D6 expression in HAP-1 cells using anti-CYP2D6 antibody (Cat#2060, 1：2000). Green, isotype control; red, CYP2D6.
Immunocytochemical staining of HAP1 cells with anti-CYP2D6 antibody (Cat #2060, 1:1,000) . Nuclei were stained blue with DAPI; CYP2D6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-CYP2D6 antibody (Cat#2060). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CYP2D6 antibody (Cat#2060, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    CYP2D6
  • 货号：
    2060
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-CYP2D6 Rabbit Monoclonal Antibody
Aliases CYP2D6; Cytochrome P450 Family 2 Subfamily D Member 6; P450-DB1; P450C2D; CYP2DL1; CYP2D; CPD6; Cytochrome P450, Subfamily IID (Debrisoquine, Sparteine, Etc., -Metabolising), Polypeptide 8 Pseudogene 2; Cytochrome P450, Subfamily II (Debrisoquine, Sparteine, Etc., -Metabolising), Polypeptide 7 Pseudogene 2; Cytochrome P450, Subfamily IID (Debrisoquine, Sparteine, Etc., -Metabolizing), Polypeptide 6; Cytochrome P450, Family 2, Subfamily D, Polypeptide 7 Pseudogene 2; Cytochrome P450, Family 2, Subfamily D, Polypeptide 8 Pseudogene 2; Cytochrome P450, Family 2, Subfamily D, Polypeptide 6; Cholesterol 25-Hydroxylase; Debrisoquine 4-Hydroxylase; Cytochrome P450 2D6; Cytochrome P450-DB1; CYP2D7AP; CYP2D7BP; CYP2D7P2; CYP2D8P2; CYPIID6; Cytochrome P450, Subfamily IID (Debrisoquine, Sparteine, Etc., -Metabolizing)-Like 1; Flavoprotein-Linked Monooxygenase; Nonfunctional Cytochrome P450 2D6; Microsomal Monooxygenase; Xenobiotic Monooxygenase; EC 1.14.14.-; EC 1.14.14.1; P450DB1
Background

Gene Name: CYP2D6

NCBI Gene Entry: 1565

UniProt Entry: P10635
Application Information

Molecular Weight: Predicted, 56 kDa; observed, 50,80 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB5800

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Cytochrome P450 2D6
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of CYP2D6 expression in HAP-1 cells using anti-CYP2D6 antibody (Cat#2060, 1：2000). Green, isotype control; red, CYP2D6.
  • Immunocytochemical staining of HAP1 cells with anti-CYP2D6 antibody (Cat #2060, 1:1,000) . Nuclei were stained blue with DAPI; CYP2D6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
  • Western blotting analysis using anti-CYP2D6 antibody (Cat#2060). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CYP2D6 antibody (Cat#2060, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    CYP2D6
  • 货号：
    2060
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Flow cytometric analysis of CYP2D6 expression in HAP-1 cells using anti-CYP2D6 antibody (Cat#2060, 1：2000). Green, isotype control; red, CYP2D6.
Immunocytochemical staining of HAP1 cells with anti-CYP2D6 antibody (Cat #2060, 1:1,000) . Nuclei were stained blue with DAPI; CYP2D6 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-CYP2D6 antibody (Cat#2060). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CYP2D6 antibody (Cat#2060, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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