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Anti-Coagulation factor IX Rabbit Monoclonal Antibody #2061

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Flow cytometric analysis of Coagulation factor IX expression in HepG2 cells using Coagulation factor IX antibody (2061, 1:2,000). Green, isotype control; red, Coagulation factor IX.

Immunocytochemical staining of HepG2 cells with anti-Coagulation factor IX antibody (Cat#2061, 1:1,000). Nuclei were stained blue with DAPI; Coagulation factor IX was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Coagulation factor IXantibody (Cat#2061). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Coagulation factor IX antibody (Cat#2061, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

F9
货号：

2061
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-Coagulation factor IX Rabbit Monoclonal Antibody
Aliases	F9; Coagulation Factor IX; FIX; Plasma Thromboplastic Component; Plasma Thromboplastin Component; Christmas Factor; EC 3.4.21.22; PTC; Christmas Disease; Hemophilia B; Factor IX F9; Factor IX; EC 3.4.21; Factor 9; F9 P22; THPH8; HEMB; P19
Background	Gene Name: F9 NCBI Gene Entry: 2158 UniProt Entry: P00740
Application Information	Molecular Weight: Predicted, 52 kDa; observed, 52 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB5805 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Factor IX
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Coagulation factor IX expression in HepG2 cells using Coagulation factor IX antibody (2061, 1:2,000). Green, isotype control; red, Coagulation factor IX.
Immunocytochemical staining of HepG2 cells with anti-Coagulation factor IX antibody (Cat#2061, 1:1,000). Nuclei were stained blue with DAPI; Coagulation factor IX was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Coagulation factor IXantibody (Cat#2061). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Coagulation factor IX antibody (Cat#2061, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

F9
货号：

2061
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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