Flow cytometric analysis of FHL2 expression in HT-1080 cells using FHL2 antibody (Cat#2072, 1:2,000). Green, isotype control; red, FHL2.
Immunocytochemical staining of HT-1080 cells with anti-FHL2 antibody (Cat #2072, 1:1,000) . Nuclei were stained blue with DAPI; FHL2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-FHL2 antibody (Cat#2072). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FHL2 antibody (Cat#2072, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
FHL2; Four And A Half LIM Domains 2; SLIM3; DRAL; Four And A Half LIM Domains Protein; Skeletal Muscle LIM-Protein 3; LIM Domain Protein DRAL; SLIM-3; FHL-2; Down-Regulated In Rhabdomyosarcoma LIM Protein; Aging-Associated Gene 11; AAG11
Flow cytometric analysis of FHL2 expression in HT-1080 cells using FHL2 antibody (Cat#2072, 1:2,000). Green, isotype control; red, FHL2.
Immunocytochemical staining of HT-1080 cells with anti-FHL2 antibody (Cat #2072, 1:1,000) . Nuclei were stained blue with DAPI; FHL2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-FHL2 antibody (Cat#2072). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FHL2 antibody (Cat#2072, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of FHL2 expression in HT-1080 cells using FHL2 antibody (Cat#2072, 1:2,000). Green, isotype control; red, FHL2.
Immunocytochemical staining of HT-1080 cells with anti-FHL2 antibody (Cat #2072, 1:1,000) . Nuclei were stained blue with DAPI; FHL2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-FHL2 antibody (Cat#2072). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FHL2 antibody (Cat#2072, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
