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Anti-EIF3E Rabbit Monoclonal Antibody #2083

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Flow cytometric analysis of EIF3E expression in HeLa cells using EIF3E antibody (Cat#2083, 1:2,000). Green, isotype control; red, EIF3E.

Immunocytochemical staining of HeLa cells with anti-EIF3E antibody (Cat #2083, 1:1,000) . Nuclei were stained blue with DAPI; EIF3E was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium . Scale bar, 20 μm.

Western blotting analysis using anti-EIF3E antibody (Cat#2083). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EIF3E antibody (Cat#2083, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

EIF3E
货号：

2083
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-EIF3E Rabbit Monoclonal Antibody
Aliases	EIF3E; Eukaryotic Translation Initiation Factor 3 Subunit E; EIF3-P48; EIF3S6; INT6; Eukaryotic Translation Initiation Factor 3, Subunit 6 48kDa; Eukaryotic Translation Initiation Factor 3 Subunit 6; Viral Integration Site Protein INT-6 Homolog; EIF-3 P48; Eukaryotic Translation Initiation Factor 3, Subunit 6 (48kD); Murine Mammary Tumor Integration Site 6 (Oncogene Homolog); Eukaryotic Translation Initiation Factor 3, Subunit E; Mammary Tumor-Associated Protein INT6; EIF3-P46; EIF3e
Background	Gene Name: EIF3E NCBI Gene Entry: 3646 UniProt Entry: P60228
Application Information	Molecular Weight: Predicted, 52 kDa; observed, 45 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB5915 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human eIF3e
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of EIF3E expression in HeLa cells using EIF3E antibody (Cat#2083, 1:2,000). Green, isotype control; red, EIF3E.
Immunocytochemical staining of HeLa cells with anti-EIF3E antibody (Cat #2083, 1:1,000) . Nuclei were stained blue with DAPI; EIF3E was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium . Scale bar, 20 μm.
Western blotting analysis using anti-EIF3E antibody (Cat#2083). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-EIF3E antibody (Cat#2083, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

EIF3E
货号：

2083
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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