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Anti-CaMKII Rabbit Monoclonal Antibody #2084

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Flow cytometric analysis of CaMKII expression in HepG2 cells using CaMKII antibody (Cat#2084, 1:2,000). Green, isotype control; red, CaMKII.
Immunocytochemical staining of HepG2 cells with anti-CaMKII antibody (Cat#2084, 1:1,000) . Nuclei were stained blue with DAPI; CaMKII was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-CaMKII antibody (Cat#2084). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CaMKII antibody (Cat#2084, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    CAMK2A/2B/2G/2D
  • 货号:
    2084
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-CaMKII Rabbit Monoclonal Antibody
Aliases CAMK2A; Calcium/Calmodulin Dependent Protein Kinase II Alpha; CaMKIINalpha; KIAA0968; CAMKA; Calcium/Calmodulin-Dependent Protein Kinase (CaM Kinase) II Alpha; Calcium/Calmodulin-Dependent Protein Kinase Type II Subunit Alpha; Calcium/Calmodulin-Dependent Protein Kinase Type II Alpha Chain; Calcium/Calmodulin-Dependent Protein Kinase II Alpha-B Subunit; CaM Kinase II Alpha Subunit; CaM Kinase II Subunit Alpha; CaM-Kinase II Alpha Chain; CaMK-II Alpha Subunit; CaMK-II Subunit Alpha; EC 2.7.11.17; CaMKIIalpha; EC 2.7.11; CaMKIIα; CAMKIIA; MRD53; MRT63; CAMK2B; Calcium/Calmodulin Dependent Protein Kinase II Beta; CAMK2; CAM2; CAMKB; Calcium/Calmodulin-Dependent Protein Kinase Type II Subunit Beta; Proline Rich Calmodulin-Dependent Protein Kinase; CaM Kinase II Beta Subunit; CaM-Kinase II Beta Chain; CaMK-II Subunit Beta; EC 2.7.11.17; Calcium/Calmodulin-Dependent Protein Kinase (CaM Kinase) II Beta; Calcium/Calmodulin-Dependent Protein Kinase Type II Beta Chain; CaM Kinase II Subunit Beta; CaMKIIbeta; EC 2.7.11; CaMKIIβ; CAMKIIB; MRD54
Background

Gene Name: CAMK2A/2B/2G/2D

NCBI Gene Entry: 815/816/818/817

UniProt Entry: Q9UQM7/Q13554/Q13555/Q13557

Application Information

Molecular Weight: Predicted, 54 kDa; observed, 50 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB5920

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human CaMKII
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of CaMKII expression in HepG2 cells using CaMKII antibody (Cat#2084, 1:2,000). Green, isotype control; red, CaMKII.
  • Immunocytochemical staining of HepG2 cells with anti-CaMKII antibody (Cat#2084, 1:1,000) . Nuclei were stained blue with DAPI; CaMKII was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
  • Western blotting analysis using anti-CaMKII antibody (Cat#2084). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CaMKII antibody (Cat#2084, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of CaMKII expression in HepG2 cells using CaMKII antibody (Cat#2084, 1:2,000). Green, isotype control; red, CaMKII.
Immunocytochemical staining of HepG2 cells with anti-CaMKII antibody (Cat#2084, 1:1,000) . Nuclei were stained blue with DAPI; CaMKII was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-CaMKII antibody (Cat#2084). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CaMKII antibody (Cat#2084, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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