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Anti-Homeostatic iron regulator Rabbit Monoclonal Antibody #2092

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Flow cytometric analysis of Homeostatic iron regulator expression in HepG2 cells using Homeostatic iron regulator antibody (Cat#2092, 1:2,000). Green, isotype control; red, Homeostatic iron regulator.

Immunocytochemical staining of HepG2 cells with anti-Homeostatic iron regulator antibody (Cat#2092, 1:1,000). Nuclei were stained blue with DAPI; Homeostatic iron regulator was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Homeostatic iron regulator antibody (Cat#2092). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Homeostatic iron regulator antibody (Cat#2092, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

HFE
货号：

2092
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-Homeostatic iron regulator Rabbit Monoclonal Antibody
Aliases	HFE; Homeostatic Iron Regulator; HLA-H; HFE1; Hereditary Hemochromatosis Protein; High Fe; Hereditary Hemochromatosis Protein HLA-H; MHC Class I-Like Protein HFE; Hemochromatosis; TFQTL2; MVCD7; HLAH; HH
Background	Gene Name: HFE NCBI Gene Entry: 3077 UniProt Entry: Q30201
Application Information	Molecular Weight: Predicted, 40 kDa; observed, 40 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB5960 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human HFE
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Homeostatic iron regulator expression in HepG2 cells using Homeostatic iron regulator antibody (Cat#2092, 1:2,000). Green, isotype control; red, Homeostatic iron regulator.
Immunocytochemical staining of HepG2 cells with anti-Homeostatic iron regulator antibody (Cat#2092, 1:1,000). Nuclei were stained blue with DAPI; Homeostatic iron regulator was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Homeostatic iron regulator antibody (Cat#2092). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Homeostatic iron regulator antibody (Cat#2092, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

HFE
货号：

2092
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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