Flow cytometric analysis of IGF2R expression in HepG2 cells using anti-IGF2R antibody (Cat#2119, 1：2000). Green, isotype control; red, IGF2R.
Immunocytochemical staining of HepG2 cells with anti-IGF2R antibody (Cat#2119, 1:1,000) . Nuclei were stained blue with DAPI; IGF2R was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-IGF2R antibody (Cat#2119). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-IGF2R antibody (Cat#2119, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of IGF2R expression in HepG2 cells using anti-IGF2R antibody (Cat#2119, 1：2000). Green, isotype control; red, IGF2R.
Immunocytochemical staining of HepG2 cells with anti-IGF2R antibody (Cat#2119, 1:1,000) . Nuclei were stained blue with DAPI; IGF2R was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-IGF2R antibody (Cat#2119). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-IGF2R antibody (Cat#2119, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of IGF2R expression in HepG2 cells using anti-IGF2R antibody (Cat#2119, 1：2000). Green, isotype control; red, IGF2R.
Immunocytochemical staining of HepG2 cells with anti-IGF2R antibody (Cat#2119, 1:1,000) . Nuclei were stained blue with DAPI; IGF2R was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-IGF2R antibody (Cat#2119). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-IGF2R antibody (Cat#2119, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
