Anti-Histone H3.3 Rabbit Monoclonal Antibody - 产品中心

Anti-Histone H3.3 Rabbit Monoclonal Antibody #2120

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Flow cytometric analysis of Histone H3.3 expression in HepG2 cells using Histone H3.3 antibody (Cat#2120, 1:2,000). Green, isotype control; red, Histone H3.3.

Immunocytochemical staining of HepG2 cells with anti-Histone H3.3 antibody (Cat#2120, 1:1,000). Nuclei were stained blue with DAPI; Histone H3.3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using anti-histone H3.3 antibody (Cat#2120, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

Western blotting analysis using anti-Histone H3.3 antibody (Cat#2120). Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Histone H3.3 antibody (Cat#2120, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

H3-3A
货号：

2120
应用：

WB, FCM, IC, IHC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-Histone H3.3 Rabbit Monoclonal Antibody
Aliases	H3-3A; H3.3 Histone A; H3.3A; H3F3A; H3F3; H3 Histone Family Member 3A; H3 Histone, Family 3A; Histone H3.3; BRYLIB1; H3-3B; H3.3B; H3F3B
Background	Gene Name: H3-3A NCBI Gene Entry: 3020 UniProt Entry: P84243
Application Information	Molecular Weight: Predicted, 15 kDa; observed, 17 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB6095 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC), immunohistochemistry (IHC)
Immunogen	A synthesized peptide derived from human Histone H3.3
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000 Immunohistochemistry (IHC): 1:100-1:200
Note	This product is for research use only.

Picture

Flow cytometric analysis of Histone H3.3 expression in HepG2 cells using Histone H3.3 antibody (Cat#2120, 1:2,000). Green, isotype control; red, Histone H3.3.
Immunocytochemical staining of HepG2 cells with anti-Histone H3.3 antibody (Cat#2120, 1:1,000). Nuclei were stained blue with DAPI; Histone H3.3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using anti-histone H3.3 antibody (Cat#2120, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Western blotting analysis using anti-Histone H3.3 antibody (Cat#2120). Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Histone H3.3 antibody (Cat#2120, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

H3-3A
货号：

2120
应用：

WB, FCM, IC, IHC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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