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Anti-GTF2E1 Rabbit Monoclonal Antibody #2164

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Flow cytometric analysis of GTF2E1 expression in H9c2 cells using GTF2E1 antibody (Cat#2164, 1:2,000). Green, isotype control; red, GTF2E1.

Immunocytochemical staining of H9C2 cells with anti-GTF2E1 antibody (Cat#2164, 1:1,000) . Nuclei were stained blue with DAPI; GTF2E1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-GTF2E1 antibody (Cat#2164). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GTF2E1 antibody (Cat#2164, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

GTF2E1
货号：

2164
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-GTF2E1 Rabbit Monoclonal Antibody
Aliases	GTF2E1; General Transcription Factor IIE Subunit 1; TFIIE-A; FE; General Transcription Factor IIE, Polypeptide 1, Alpha 56kDa; Transcription Initiation Factor IIE Subunit Alpha; General Transcription Factor IIE 56 KDa Subunit; TFIIE Alpha Subunit; TFIIE-Alpha; TF2E1; General Transcription Factor IIE, Polypeptide 1 (Alpha Subunit, 56kD)
Background	Gene Name: GTF2E1 NCBI Gene Entry: 2960 UniProt Entry: P29083
Application Information	Molecular Weight: Predicted, 49 kDa; observed, 60 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB6310 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human TFIIE alpha
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of GTF2E1 expression in H9c2 cells using GTF2E1 antibody (Cat#2164, 1:2,000). Green, isotype control; red, GTF2E1.
Immunocytochemical staining of H9C2 cells with anti-GTF2E1 antibody (Cat#2164, 1:1,000) . Nuclei were stained blue with DAPI; GTF2E1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-GTF2E1 antibody (Cat#2164). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GTF2E1 antibody (Cat#2164, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

GTF2E1
货号：

2164
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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