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Anti-hnRNP U Rabbit Monoclonal Antibody #2173

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Flow cytometric analysis of HNRNPU expression in HepG2 cells using HNRNPU antibody (Cat#2173, 1:2,000). Green, isotype control; red, HNRNPU.
Immunocytochemical staining of HepG2 cells with anti-HNRNPU antibody (Cat #2173, 1:1,000) . Nuclei were stained blue with DAPI; HNRNPU was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-HNRNPU antibody (Cat#2173). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HNRNPU antibody (Cat#2173, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    HNRNPU
  • 货号:
    2173
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-hnRNP U Rabbit Monoclonal Antibody
Aliases HNRNPU; Heterogeneous Nuclear Ribonucleoprotein U; SAF-A; HNRPU; Heterogeneous Nuclear Ribonucleoprotein U (Scaffold Attachment Factor A); Nuclear P120 Ribonucleoprotein; HNRNPU Antisense RNA 1; HNRNPU-AS1; FLJ37978; FLJ30202; GRIP120; HnRNP U; U21.1; Pp120; SAFA; HNRNPU Antisense RNA 1 (Non-Protein Coding); Chromosome 1 Open Reading Frame 199; Scaffold Attachment Factor A; Scaffold-Attachment Factor A; Non-Protein Coding RNA 201; P120 Nuclear Protein; NCRNA00201; C1ORF199; C1orf199; EIEE54; DEE54; P120
Background

Gene Name: HNRNPU

NCBI Gene Entry: 3192

UniProt Entry: Q00839

Application Information

Molecular Weight: Predicted, 91 kDa; observed, 120 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB6350

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human hnRNP U
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of HNRNPU expression in HepG2 cells using HNRNPU antibody (Cat#2173, 1:2,000). Green, isotype control; red, HNRNPU.
  • Immunocytochemical staining of HepG2 cells with anti-HNRNPU antibody (Cat #2173, 1:1,000) . Nuclei were stained blue with DAPI; HNRNPU was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
  • Western blotting analysis using anti-HNRNPU antibody (Cat#2173). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HNRNPU antibody (Cat#2173, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of HNRNPU expression in HepG2 cells using HNRNPU antibody (Cat#2173, 1:2,000). Green, isotype control; red, HNRNPU.
Immunocytochemical staining of HepG2 cells with anti-HNRNPU antibody (Cat #2173, 1:1,000) . Nuclei were stained blue with DAPI; HNRNPU was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-HNRNPU antibody (Cat#2173). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HNRNPU antibody (Cat#2173, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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