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Anti-CCR7 Rabbit Monoclonal Antibody #2202

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Flow cytometric analysis of CCR7 expression in HepG2 cells using CCR7 antibody (Cat#2202, 1:2,000). Green, isotype control; red, CCR7.
Immunocytochemical staining of HepG2 cells with anti-CCR7 antibody (Cat#2202, 1:1,000). Nuclei were stained blue with DAPI; CCR7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-CCR7 antibody (Cat#2202). Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CCR7 antibody (Cat#2202, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    CCR7
  • 货号:
    2202
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-CCR7 Rabbit Monoclonal Antibody
Aliases CCR7; C-C Motif Chemokine Receptor 7; CDw197; BLR2; CMKBR7; CD197; EBI1; Epstein-Barr Virus-Induced G-Protein Coupled Receptor 1; Chemokine (C-C Motif) Receptor 7; C-C Chemokine Receptor Type 7; MIP-3 Beta Receptor; CC-CKR-7; CCR-7; Lymphocyte-Specific G Protein-Coupled Peptide Receptor; EBV-Induced G Protein-Coupled Receptor 1; EBV-Induced G-Protein Coupled Receptor 1; Epstein-Barr Virus Induced Gene 1; Bukitt'S Lymphoma Receptor; CC Chemokine Receptor 7; CD197 Antigen; C-C CKR-7; EVI1
Background

Gene Name: CCR7

NCBI Gene Entry: 1236

UniProt Entry: P32248

Application Information

Molecular Weight: Predicted, 43 kDa; observed, 43 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB6485

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human CCR7
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of CCR7 expression in HepG2 cells using CCR7 antibody (Cat#2202, 1:2,000). Green, isotype control; red, CCR7.
  • Immunocytochemical staining of HepG2 cells with anti-CCR7 antibody (Cat#2202, 1:1,000). Nuclei were stained blue with DAPI; CCR7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-CCR7 antibody (Cat#2202). Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CCR7 antibody (Cat#2202, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of CCR7 expression in HepG2 cells using CCR7 antibody (Cat#2202, 1:2,000). Green, isotype control; red, CCR7.
Immunocytochemical staining of HepG2 cells with anti-CCR7 antibody (Cat#2202, 1:1,000). Nuclei were stained blue with DAPI; CCR7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-CCR7 antibody (Cat#2202). Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CCR7 antibody (Cat#2202, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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