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Anti-IDH2 Rabbit Monoclonal Antibody #2223

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Flow cytometric analysis of IDH2 expression in C2C12 cells using IDH2 antibody (Cat#2223, 1:2,000). Green, isotype control; red, IDH2.

Immunocytochemical staining of C2C12 cells with anti-IDH2 antibody (Cat#2223, 1:1,000). Nuclei were stained blue with DAPI; IDH2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using anti-IDH2 antibody (Cat#2223). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-IDH2 antibody (Cat#2223, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

IDH2
货号：

2223
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Information

Product Name	Anti-IDH2 Rabbit Monoclonal Antibody
Aliases	IDH2; Isocitrate Dehydrogenase (NADP(+)) 2; IDH-2; Isocitrate Dehydrogenase (NADP(+)) 2, Mitochondrial; Isocitrate Dehydrogenase 2 (NADP+), Mitochondrial; Isocitrate Dehydrogenase [NADP], Mitochondrial; Oxalosuccinate Decarboxylase; NADP(+)-Specific ICDH; EC 1.1.1.42; ICD-M; IDH; IDP; MNADP-IDH; D2HGA2; IDHM; IDPM
Background	Gene Name: IDH2 NCBI Gene Entry: 3418 UniProt Entry: P48735
Application Information	Molecular Weight: Predicted, 51 kDa; observed, 45 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB6580 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human IDH2
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of IDH2 expression in C2C12 cells using IDH2 antibody (Cat#2223, 1:2,000). Green, isotype control; red, IDH2.
Immunocytochemical staining of C2C12 cells with anti-IDH2 antibody (Cat#2223, 1:1,000). Nuclei were stained blue with DAPI; IDH2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-IDH2 antibody (Cat#2223). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-IDH2 antibody (Cat#2223, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

IDH2
货号：

2223
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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