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Anti-LFNG Rabbit Monoclonal Antibody #2227

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Flow cytometric analysis of LFNG expression in HepG2 cells using LFNG antibody (Cat#2227, 1:2,000). Green, isotype control; red, LFNG.

Immunocytochemical staining of HepG2 cells with anti-LFNG antibody (Cat #2227, 1:1,000). Nuclei were stained blue with DAPI; LFNG was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-LFNG antibody (Cat#2227). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-LFNG antibody (Cat#2227, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

LFNG
货号：

2227
应用：

WB, FCM, IC
物种反应性：

H, M

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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Information

Product Name	Anti-LFNG Rabbit Monoclonal Antibody
Aliases	LFNG; LFNG O-Fucosylpeptide 3-Beta-N-Acetylglucosaminyltransferase; SCDO3; Beta-1,3-N-Acetylglucosaminyltransferase Lunatic Fringe; EC 2.4.1.222; O-Fucosylpeptide 3-Beta-N-Acetylglucosaminyltransferase; Lunatic Fringe (Drosophila) Homolog; Lunatic Fringe Homolog (Drosophila)
Background	Gene Name: LFNG NCBI Gene Entry: 3955 UniProt Entry: Q8NES3
Application Information	Molecular Weight: Predicted, 42 kDa; observed, 42 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB6595 Species Reactivity: Human, mouse Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Lunatic Fringe
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of LFNG expression in HepG2 cells using LFNG antibody (Cat#2227, 1:2,000). Green, isotype control; red, LFNG.
Immunocytochemical staining of HepG2 cells with anti-LFNG antibody (Cat #2227, 1:1,000). Nuclei were stained blue with DAPI; LFNG was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-LFNG antibody (Cat#2227). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-LFNG antibody (Cat#2227, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

LFNG
货号：

2227
应用：

WB, FCM, IC
物种反应性：

H, M

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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