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Anti-GPRC5B Rabbit Monoclonal Antibody #2256

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Flow cytometric analysis of GPRC5B expression in HepG2 cells using GPRC5B antibody (Cat#2256, 1:2,000). Green, isotype control; red, GPRC5B.

Immunocytochemical staining of HepG2 cells with anti-GPRC5B antibody (Cat#2256, 1:1000) . Nuclei were stained blue with DAPI; GPRC5B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-GPRC5B antibody (Cat#2256). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GPRC5B antibody (Cat#2256, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

GPRC5B
货号：

2256
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-GPRC5B Rabbit Monoclonal Antibody
Aliases	GPRC5B; G Protein-Coupled Receptor Class C Group 5 Member B; RAIG-2; G Protein-Coupled Receptor, Family C, Group 1, Member B; G-Protein Coupled Receptor Family C Group 5 Member B; Retinoic Acid-Induced Gene 2 Protein; RAIG2; G Protein-Coupled Receptor, Family C, Group 5, Member B; G Protein-Coupled Receptor, Class C, Group 5, Member B; Retinoic Acid Responsive Gene Protein; A-69G12.1; MLC3
Background	Gene Name: GPRC5B NCBI Gene Entry: 51704 UniProt Entry: Q9NZH0
Application Information	Molecular Weight: Predicted, 45 kDa; observed, 52 kDa Clonality: Rabbit monoclonal antibody Clone ID: 23GB5710 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human GPRC5B
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of GPRC5B expression in HepG2 cells using GPRC5B antibody (Cat#2256, 1:2,000). Green, isotype control; red, GPRC5B.
Immunocytochemical staining of HepG2 cells with anti-GPRC5B antibody (Cat#2256, 1:1000) . Nuclei were stained blue with DAPI; GPRC5B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-GPRC5B antibody (Cat#2256). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GPRC5B antibody (Cat#2256, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

GPRC5B
货号：

2256
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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