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Anti-CDH1 Rabbit Monoclonal Antibody #2257

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Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using anti-CDH1 antibody (Cat#2257, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Western blotting analysis using anti-CDH1 antibody (Cat#2257). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CDH1 antibody (Cat#2257, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    CDH1
  • 货号：
    2257
  • 应用：
    WB, IHC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information
Product Name Anti-CDH1 Rabbit Monoclonal Antibody
Aliases CDH1; Cadherin 1; Uvomorulin; CD324; UVO; Cadherin 1, Type 1, E-Cadherin (Epithelial); Epithelial Cadherin; E-Cadherin; Cadherin-1; CAM 120/80; CDHE; Calcium-Dependent Adhesion Protein, Epithelial; Epididymis Secretory Sperm Binding Protein; Cadherin 1, E-Cadherin (Epithelial); Cell-CAM 120/80; CD324 Antigen; E-Cadherin 1; UVOMORULIN; Arc-1; BCDS1; ECAD; LCAM
Background

Gene Name: CDH1

NCBI Gene Entry: 999

UniProt Entry: P12830
Application Information

Molecular Weight: Predicted, 97 kDa; observed, 135 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB5715

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunohistochemistry (IHC)
Immunogen A synthesized peptide derived from human E Cadherin
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunohistochemistry (IHC): 1:100-1:200
Note This product is for research use only.
Picture
  • Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using anti-CDH1 antibody (Cat#2257, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Western blotting analysis using anti-CDH1 antibody (Cat#2257). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CDH1 antibody (Cat#2257, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    CDH1
  • 货号：
    2257
  • 应用：
    WB, IHC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using anti-CDH1 antibody (Cat#2257, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Western blotting analysis using anti-CDH1 antibody (Cat#2257). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CDH1 antibody (Cat#2257, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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