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Anti-5'-Nucleotidase Ecto Rabbit Monoclonal Antibody #2339

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Flow cytometric analysis of CD73 expression in HepG2 cells using anti-CD73 antibody (Cat#2339, 1:2,000). Green, isotype control; red, CD73.

Immunocytochemical staining of HepG2 cells with anti-CD73 antibody (Cat#2339, 1:1,000). Nuclei were stained blue with DAPI; CD73 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-CD73 antibody (Cat#2339). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD73 antibody (Cat#2339, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

NT5E
货号：

2339
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-5'-Nucleotidase Ecto Rabbit Monoclonal Antibody
Aliases	5'-Nucleotidase Ecto; CALJA; CD73; ENT; NT5; EN; IMP-Specific 5'-Nucleotidase; Thymidylate 5'-Phosphatase; 5'-Deoxynucleotidase; Ecto-5'-Nucleotidase; 5'-Nucleotidase; EC 3.1.3.5; 5'-NT; NTE; 5'-Nucleotidase, Ecto (CD73); Purine 5-Prime-Nucleotidase; 5' Nucleotidase (CD73); CD73 Antigen; EC 3.1.3.35; EC 3.1.3.89; EC 3.1.3.91; EC 3.1.3.99; E5NT; NT
Background	Gene Name: NT5E NCBI Gene Entry: 4907 UniProt Entry: P21589
Application Information	Molecular Weight: Predicted, 63 kDa; observed, 70 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB375 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human CD73
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of CD73 expression in HepG2 cells using anti-CD73 antibody (Cat#2339, 1:2,000). Green, isotype control; red, CD73.
Immunocytochemical staining of HepG2 cells with anti-CD73 antibody (Cat#2339, 1:1,000). Nuclei were stained blue with DAPI; CD73 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-CD73 antibody (Cat#2339). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD73 antibody (Cat#2339, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

NT5E
货号：

2339
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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