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Anti-NDC80 Rabbit Monoclonal Antibody #2348

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Flow cytometric analysis of NDC80 expression in HepG2 cells using NDC80 antibody (Cat#2348, 1:2,000). Green, isotype control; red, NDC80.

Immunocytochemical staining of HepG2 cells with anti-NDC80 antibody (Cat#2348, 1:1,000). Nuclei were stained blue with DAPI; NDC80 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.

Western blotting analysis using anti-NDC80 antibody (Cat#2348). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NDC80 antibody (Cat#2348, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

NDC80
货号：

2348
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-NDC80 Rabbit Monoclonal Antibody
Aliases	NDC80 Kinetochore Complex Component; HEC1; HEC; HsNDC80; KNTC2; TID3; Retinoblastoma-Associated Protein HEC; Highly Expressed In Cancer Protein; Kinetochore Protein NDC80 Homolog; Kinetochore-Associated Protein 2; Kinetochore Associated 2; Kinetochore Protein Hec1; HsHec1; Highly Expressed In Cancer, Rich In Leucine Heptad Repeats (Yeast); NDC80 Kinetochore Complex Component Homolog (S. Cerevisiae); Highly Expressed In Cancer, Rich In Leucine Heptad Repeats; NDC80 Homolog, Kinetochore Complex Component; NDC80 Kinetochore Complex Component Homolog
Background	Gene Name: NDC80 NCBI Gene Entry: 10403 UniProt Entry: O14777
Application Information	Molecular Weight: Predicted, 74 kDa; observed, 74 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB415 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human HEC1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of NDC80 expression in HepG2 cells using NDC80 antibody (Cat#2348, 1:2,000). Green, isotype control; red, NDC80.
Immunocytochemical staining of HepG2 cells with anti-NDC80 antibody (Cat#2348, 1:1,000). Nuclei were stained blue with DAPI; NDC80 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-NDC80 antibody (Cat#2348). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NDC80 antibody (Cat#2348, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

NDC80
货号：

2348
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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