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Anti-PAF1 Rabbit Monoclonal Antibody #2392

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Immunocytochemical staining of C2C12 cells with anti-PAF1 antibody (Cat#2392, 1:1,000). Nuclei were stained blue with DAPI; PAF1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-PAF1 antibody (Cat#2392). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PAF1 antibody (Cat#2392, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    PAF1
  • 货号：
    2392
  • 应用：
    WB, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-PAF1 Rabbit Monoclonal Antibody
Aliases PAF1 Homolog, Paf1/RNA Polymerase II Complex Component; PD2; F23149_1; RNA Polymerase II-Associated Factor 1 Homolog; Pancreatic Differentiation Protein 2; FLJ11123; Paf1, RNA Polymerase II Associated Factor, Homolog (S. Cerevisiae); Paf1, RNA Polymerase II Associated Factor, Homolog; HPAF1
Background

Gene Name: PAF1

NCBI Gene Entry: 54623

UniProt Entry: Q8N7H5
Application Information

Molecular Weight: Predicted, 60 kDa; observed, 80 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB625

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human PAF1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Immunocytochemical staining of C2C12 cells with anti-PAF1 antibody (Cat#2392, 1:1,000). Nuclei were stained blue with DAPI; PAF1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-PAF1 antibody (Cat#2392). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PAF1 antibody (Cat#2392, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    PAF1
  • 货号：
    2392
  • 应用：
    WB, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Immunocytochemical staining of C2C12 cells with anti-PAF1 antibody (Cat#2392, 1:1,000). Nuclei were stained blue with DAPI; PAF1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-PAF1 antibody (Cat#2392). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PAF1 antibody (Cat#2392, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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