Flow cytometric analysis of NCK1 expression in HT-1080 cells using NCK1 antibody (Cat#2408, 1:2,000). Green, isotype control; red, NCK1.
Immunocytochemical staining of HT-1080 cells with anti-NCK1 antibody (Cat#2408, 1:1,000). Nuclei were stained blue with DAPI; NCK1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-NCK1 antibody (Cat#2408). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NCK1 antibody (Cat#2408, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Anti-NCK Adaptor Protein 1 Rabbit Monoclonal Antibody
Aliases
NCK Adaptor Protein 1; NCKalpha; NCK; SH2/SH3 Adaptor Protein NCK-Alpha; Cytoplasmic Protein NCK1; Nck-1; Non-Catalytic Region Of Tyrosine Kinase; Melanoma NCK Protein; NCK Tyrosine Kinase
Flow cytometric analysis of NCK1 expression in HT-1080 cells using NCK1 antibody (Cat#2408, 1:2,000). Green, isotype control; red, NCK1.
Immunocytochemical staining of HT-1080 cells with anti-NCK1 antibody (Cat#2408, 1:1,000). Nuclei were stained blue with DAPI; NCK1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-NCK1 antibody (Cat#2408). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NCK1 antibody (Cat#2408, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of NCK1 expression in HT-1080 cells using NCK1 antibody (Cat#2408, 1:2,000). Green, isotype control; red, NCK1.
Immunocytochemical staining of HT-1080 cells with anti-NCK1 antibody (Cat#2408, 1:1,000). Nuclei were stained blue with DAPI; NCK1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-NCK1 antibody (Cat#2408). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NCK1 antibody (Cat#2408, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
