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Anti-NDUFAF1 Rabbit Monoclonal Antibody #2415

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Flow cytometric analysis of NDUFAF1 expression in HeLa cells using NDUFAF1 antibody (Cat#2415, 1:2,000). Green, isotype control; red, NDUFAF1.

Immunocytochemical staining of HeLa cells with anti-NDUFAF1 antibody (Cat#2415, 1:1,000). Nuclei were stained blue with DAPI; NDUFAF1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-NDUFAF1 antibody (Cat#2415). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NDUFAF1 antibody (Cat#2415, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

NDUFAF1
货号：

2415
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-NDUFAF1 Rabbit Monoclonal Antibody
Aliases	NADH:Ubiquinone Oxidoreductase Complex Assembly Factor 1; CIA30; CGI-65; NADH Dehydrogenase (Ubiquinone) Complex I, Assembly Factor 1; Complex I Intermediate-Associated Protein 30, Mitochondrial; NADH Dehydrogenase (Ubiquinone) 1 Alpha Subcomplex, Assembly Factor 1; NADH-Ubiquinone Oxidoreductase 1 Alpha Subcomplex, Assembly Factor 1; NADH Dehydrogenase [Ubiquinone] 1 Alpha Subcomplex Assembly Factor 1; MC1DN11; CGI65
Background	Gene Name: NDUFAF1 NCBI Gene Entry: 51103 UniProt Entry: Q9Y375
Application Information	Molecular Weight: Predicted, 38 kDa; observed, 33 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB735 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human NDUFAF1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of NDUFAF1 expression in HeLa cells using NDUFAF1 antibody (Cat#2415, 1:2,000). Green, isotype control; red, NDUFAF1.
Immunocytochemical staining of HeLa cells with anti-NDUFAF1 antibody (Cat#2415, 1:1,000). Nuclei were stained blue with DAPI; NDUFAF1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-NDUFAF1 antibody (Cat#2415). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NDUFAF1 antibody (Cat#2415, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

NDUFAF1
货号：

2415
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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