Anti-Splicing Factor 3b Subunit 1 Rabbit Monoclonal Antibody - 产品中心

Anti-Splicing Factor 3b Subunit 1 Rabbit Monoclonal Antibody #2519

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Flow cytometric analysis of SF3B1 expression in HepG2 cells using anti-SF3B1 antibody (Cat#2519, 1:2,000). Green, isotype control; red, SF3B1.

Immunocytochemical staining of HepG2 cells with anti-SF3B1 antibody (Cat#2519, 1:1,000). Nuclei were stained blue with DAPI; SF3B1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-SF3B1 antibody (Cat#2519). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SF3B1 antibody (Cat#2519, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

SF3B1
货号：

2519
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-Splicing Factor 3b Subunit 1 Rabbit Monoclonal Antibody
Aliases	Splicing Factor 3b Subunit 1; SF3b155; SAP155; PRPF10; Hsh155; Prp10; Splicing Factor 3b, Subunit 1, 155kDa; Spliceosome-Associated Protein 155; Pre-MRNA Splicing Factor SF3b, 155 KDa Subunit; Pre-MRNA-Splicing Factor SF3b 155 KDa Subunit; Splicing Factor 3b, Subunit 1, 155kD; Splicing Factor 3B Subunit 1; Pre-MRNA Processing 10; SAP 155; MDS
Background	Gene Name: SF3B1 NCBI Gene Entry: 23451 UniProt Entry: O75533
Application Information	Molecular Weight: Predicted, 146 kDa; observed, 155 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB1225 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human SF3B1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of SF3B1 expression in HepG2 cells using anti-SF3B1 antibody (Cat#2519, 1:2,000). Green, isotype control; red, SF3B1.
Immunocytochemical staining of HepG2 cells with anti-SF3B1 antibody (Cat#2519, 1:1,000). Nuclei were stained blue with DAPI; SF3B1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-SF3B1 antibody (Cat#2519). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SF3B1 antibody (Cat#2519, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

SF3B1
货号：

2519
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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