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Anti-Regulator Of Calcineurin 1 Rabbit Monoclonal Antibody #2543

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Flow cytometric analysis of Regulator of calcineurin 1 expression in C2C12 cells using anti-Regulator of calcineurin 1 antibody (Cat#2543, 1:2,000). Green, isotype control; red, Regulator of calcineurin 1.
Immunocytochemical staining of C2C12 cells with anti-Regulator of calcineurin 1 antibody (Cat#2543, 1:1,000) . Nuclei were stained blue with DAPI; Regulator of calcineurin 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-Regulator of calcineurin 1 antibody (Cat#2543). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Regulator of calcineurin 1 antibody (Cat#2543, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    RCAN1
  • 货号:
    2543
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-Regulator Of Calcineurin 1 Rabbit Monoclonal Antibody
Aliases RCAN1; Regulator Of Calcineurin 1; DSCR1; Myocyte-Enriched Calcineurin-Interacting Protein 1; Down Syndrome Critical Region Gene 1; Calcipressin-1; ADAPT78; MCIP1; CSP1; DSC1; Modulatory Calcineurin-Interacting Protein 1; Down Syndrome Critical Region Protein 1; Calcium And Oxidant-Inducible MRNA; Down Syndrome Candidate Region 1; Near DSCR Proline-Rich Protein; Adapt78; RCN1
Background

Gene Name: RCAN1

NCBI Gene Entry: 1827

UniProt Entry: P53805

Application Information

Molecular Weight: Predicted, 28 kDa; observed, 28 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB1335

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human Calcipressin 1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Regulator of calcineurin 1 expression in C2C12 cells using anti-Regulator of calcineurin 1 antibody (Cat#2543, 1:2,000). Green, isotype control; red, Regulator of calcineurin 1.
  • Immunocytochemical staining of C2C12 cells with anti-Regulator of calcineurin 1 antibody (Cat#2543, 1:1,000) . Nuclei were stained blue with DAPI; Regulator of calcineurin 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
  • Western blotting analysis using anti-Regulator of calcineurin 1 antibody (Cat#2543). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Regulator of calcineurin 1 antibody (Cat#2543, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of Regulator of calcineurin 1 expression in C2C12 cells using anti-Regulator of calcineurin 1 antibody (Cat#2543, 1:2,000). Green, isotype control; red, Regulator of calcineurin 1.
Immunocytochemical staining of C2C12 cells with anti-Regulator of calcineurin 1 antibody (Cat#2543, 1:1,000) . Nuclei were stained blue with DAPI; Regulator of calcineurin 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-Regulator of calcineurin 1 antibody (Cat#2543). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Regulator of calcineurin 1 antibody (Cat#2543, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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