Anti-Sphingosine-1-Phosphate Receptor 1 Rabbit Monoclonal Antibody - 产品中心

Anti-Sphingosine-1-Phosphate Receptor 1 Rabbit Monoclonal Antibody #2567

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Flow cytometric analysis of Sphingosine-1-phosphate receptor 1 expression in HepG2 cells using anti-Sphingosine-1-phosphate receptor 1 antibody (Cat#2567, 1:2,000). Green, isotype control; red, Sphingosine-1-phosphate receptor 1.

Immunocytochemical staining of HepG2 cells with anti-Sphingosine-1-phosphate receptor 1 antibody (Cat#2567, 1:1,000). Nuclei were stained blue with DAPI; Sphingosine-1-phosphate receptor 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.

Western blotting analysis using anti-Sphingosine-1-phosphate receptor 1 antibody (Cat#2567). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Sphingosine-1-phosphate receptor 1 antibody (Cat#2567, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

S1PR1
货号：

2567
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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Information

Product Name	Anti-Sphingosine-1-Phosphate Receptor 1 Rabbit Monoclonal Antibody
Aliases	S1PR1; Sphingosine-1-Phosphate Receptor 1; D1S3362; Edg-1; CD363; EDG1; Endothelial Differentiation, Sphingolipid G-Protein-Coupled Receptor, 1;Endothelial Differentiation G-Protein Coupled Receptor 1 ; Sphingosine 1-Phosphate Receptor Edg-1; Sphingosine 1-Phosphate Receptor 1; S1P Receptor Edg-1; S1P Receptor 1; CHEDG1; S1P1; Sphingosine 1-Phosphate Receptor EDG1; CD363 Antigen; ECGF1
Background	Gene Name: S1PR1 NCBI Gene Entry: 1901 UniProt Entry: P21453
Application Information	Molecular Weight: Predicted, 43 kDa; observed, 43 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB1435 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human EDG1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Sphingosine-1-phosphate receptor 1 expression in HepG2 cells using anti-Sphingosine-1-phosphate receptor 1 antibody (Cat#2567, 1:2,000). Green, isotype control; red, Sphingosine-1-phosphate receptor 1.
Immunocytochemical staining of HepG2 cells with anti-Sphingosine-1-phosphate receptor 1 antibody (Cat#2567, 1:1,000). Nuclei were stained blue with DAPI; Sphingosine-1-phosphate receptor 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Sphingosine-1-phosphate receptor 1 antibody (Cat#2567). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Sphingosine-1-phosphate receptor 1 antibody (Cat#2567, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

S1PR1
货号：

2567
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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