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Anti-Sarcoglycan Alpha Rabbit Monoclonal Antibody #2572

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Flow cytometric analysis of Sarcoglycan alpha expression in C2C12 cells using anti-Sarcoglycan alpha antibody (Cat#2572, 1:2,000). Green, isotype control; red, Sarcoglycan alpha.

Immunocytochemical staining of C2C12 cells with anti-Sarcoglycan alpha antibody (Cat#2572, 1:1,000) . Nuclei were stained blue with DAPI; Sarcoglycan alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-Sarcoglycan alpha antibody (Cat#2572). Total cell lysates (10 μg for C2C12 and 30 μg for others) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Sarcoglycan alpha antibody (Cat#2572, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

SGCA
货号：

2572
应用：

WB, FCM, IC
物种反应性：

M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Information

Product Name	Anti-Sarcoglycan Alpha Rabbit Monoclonal Antibody
Aliases	SGCA; Sarcoglycan Alpha; Adhalin; SCARMD1; LGMD2D; DMDA2; ADL; Sarcoglycan, Alpha (50kDa Dystrophin-Associated Glycoprotein); 50 KDa Dystrophin-Associated Glycoprotein; Limb Girdle Muscular Dystrophy 2D; Alpha-Sarcoglycan; Dystroglycan-2; 50kD DAG; Alpha-SG; 50DAG; DAG2; A2; LGMDR3
Background	Gene Name: SGCA NCBI Gene Entry: 6442 UniProt Entry: Q16586
Application Information	Molecular Weight: Predicted, 43 kDa; observed, 50 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB1460 Species Reactivity: Mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human alpha Sarcoglycan
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Sarcoglycan alpha expression in C2C12 cells using anti-Sarcoglycan alpha antibody (Cat#2572, 1:2,000). Green, isotype control; red, Sarcoglycan alpha.
Immunocytochemical staining of C2C12 cells with anti-Sarcoglycan alpha antibody (Cat#2572, 1:1,000) . Nuclei were stained blue with DAPI; Sarcoglycan alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-Sarcoglycan alpha antibody (Cat#2572). Total cell lysates (10 μg for C2C12 and 30 μg for others) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Sarcoglycan alpha antibody (Cat#2572, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

SGCA
货号：

2572
应用：

WB, FCM, IC
物种反应性：

M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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