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Anti-S1PR3 Rabbit Monoclonal Antibody #2603

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Flow cytometric analysis of S1PR3 expression in HAP-1 cells using anti-S1PR3 antibody (Cat#2603, 1:2,000). Green, isotype control; red, S1PR3.

Immunocytochemical staining of HAP1 cells with anti-S1PR3 antibody (Cat#2603, 1:1,000) . Nuclei were stained blue with DAPI; S1PR3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-S1PR3 antibody (Cat#2603). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-S1PR3 antibody (Cat#2603, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

S1PR3
货号：

2603
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-S1PR3 Rabbit Monoclonal Antibody
Aliases	Sphingosine-1-Phosphate Receptor 3; BA791O21.3; C9orf108; C9orf47; EDG-3; EDG3; Endothelial Differentiation, Sphingolipid G-Protein-Coupled Receptor,3; Endothelial Differentiation G-Protein Coupled Receptor 3; Sphingosine 1-Phosphate Receptor Edg-3; Sphingosine 1-Phosphate Receptor 3; S1P Receptor Edg-3; S1P Receptor 3; FLJ37523; S1P3; G Protein-Coupled Receptor, Endothelial Differentiation Gene-3; Chromosome 9 Open Reading Frame 108; Chromosome 9 Open Reading Frame 47; Uncharacterized Protein C9orf47; S1P Receptor EDG3; LPB3
Background	Gene Name: S1PR3 NCBI Gene Entry: 1903 UniProt Entry: Q99500
Application Information	Molecular Weight: Predicted, 42 kDa; observed, 42 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB1605 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human S1PR3
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of S1PR3 expression in HAP-1 cells using anti-S1PR3 antibody (Cat#2603, 1:2,000). Green, isotype control; red, S1PR3.
Immunocytochemical staining of HAP1 cells with anti-S1PR3 antibody (Cat#2603, 1:1,000) . Nuclei were stained blue with DAPI; S1PR3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-S1PR3 antibody (Cat#2603). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-S1PR3 antibody (Cat#2603, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

S1PR3
货号：

2603
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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