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Anti-SMN1 Rabbit Monoclonal Antibody #2673

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Flow cytometric analysis of SMN1 expression in HAP-1 cells using anti-SMN1 antibody (Cat#2673, 1:2,000). Green, isotype control; red, SMN1.
Immunocytochemical staining of H9c2 cells with SMN1 antibody (Cat#2673, 1:1,000) . Nuclei were stained blue with DAPI; SMN1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: . Scale bar, 20 μm.
Western blotting analysis using anti-SMN1 antibody (Cat#2673). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SMN1 antibody (Cat#2673, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    SMN1
  • 货号:
    2673
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-SMN1 Rabbit Monoclonal Antibody
Aliases SMN1; Survival Of Motor Neuron 1, Telomeric; SMNT; TDRD16A; Gemin-1; BCD541; GEMIN1; SMA1; SMA2; SMA3; Survival Motor Neuron Protein; Tudor Domain Containing 16A; Component Of Gems 1; SMA@; SMA; SMN; Spinal Muscular Atrophy (Werdnig-Hoffmann Disease, Kugelberg-Welander Disease); Survival Motor Neuron 1 Protein; T-BCD541; SMA4; SMNC
Background

Gene Name: SMN1

NCBI Gene Entry: 6606

UniProt Entry: Q16637

Application Information

Molecular Weight: Predicted, 32 kDa; observed, 36 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB2535

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human SMN1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of SMN1 expression in HAP-1 cells using anti-SMN1 antibody (Cat#2673, 1:2,000). Green, isotype control; red, SMN1.
  • Immunocytochemical staining of H9c2 cells with SMN1 antibody (Cat#2673, 1:1,000) . Nuclei were stained blue with DAPI; SMN1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: . Scale bar, 20 μm.
  • Western blotting analysis using anti-SMN1 antibody (Cat#2673). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SMN1 antibody (Cat#2673, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of SMN1 expression in HAP-1 cells using anti-SMN1 antibody (Cat#2673, 1:2,000). Green, isotype control; red, SMN1.
Immunocytochemical staining of H9c2 cells with SMN1 antibody (Cat#2673, 1:1,000) . Nuclei were stained blue with DAPI; SMN1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: . Scale bar, 20 μm.
Western blotting analysis using anti-SMN1 antibody (Cat#2673). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SMN1 antibody (Cat#2673, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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