Anti-Tripartite Motif Containing 25 Rabbit Monoclonal Antibody - 产品中心

Anti-Tripartite Motif Containing 25 Rabbit Monoclonal Antibody #2713

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Flow cytometric analysis of TRIM25 expression in HepG2 cells using anti-TRIM25 antibody (Cat#2713, 1:2,000). Green, isotype control; red, TRIM25.

Immunocytochemical staining of HepG2 cells with anti-TRIM25 antibody (Cat#2713, 1:1,000) . Nuclei were stained blue with DAPI; TRIM25 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using anti-TRIM25 antibody (Cat#2713). Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TRIM25 antibody (Cat#2713, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

TRIM25
货号：

2713
应用：

WB, FCM, IC
物种反应性：

H, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-Tripartite Motif Containing 25 Rabbit Monoclonal Antibody
Aliases	TRIM25; Tripartite Motif Containing 25; RNF147; EFP; ZNF147; Zinc Finger Protein 147 (Estrogen-Responsive Finger Protein); RING-Type E3 Ubiquitin Transferase TRIM25; Ubiquitin/ISG15-Conjugating Enzyme TRIM25; Tripartite Motif-Containing Protein 25; Estrogen-Responsive Finger Protein; E3 Ubiquitin/ISG15 Ligase TRIM25; RING Finger Protein 147; RING-Type E3 Ubiquitin Transferase; Tripartite Motif Protein TRIM25; Tripartite Motif-Containing 25; Zinc Finger Protein-147; Zinc Finger Protein 147; EC 6.3.2.N3; EC 2.3.2.27; Z147
Background	Gene Name: TRIM25 NCBI Gene Entry: 7706 UniProt Entry: Q14258
Application Information	Molecular Weight: Predicted, 71 kDa; observed, 71 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB2245 Species Reactivity: Human, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human TRIM25
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of TRIM25 expression in HepG2 cells using anti-TRIM25 antibody (Cat#2713, 1:2,000). Green, isotype control; red, TRIM25.
Immunocytochemical staining of HepG2 cells with anti-TRIM25 antibody (Cat#2713, 1:1,000) . Nuclei were stained blue with DAPI; TRIM25 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-TRIM25 antibody (Cat#2713). Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TRIM25 antibody (Cat#2713, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

TRIM25
货号：

2713
应用：

WB, FCM, IC
物种反应性：

H, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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