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Anti-UBE2N Rabbit Monoclonal Antibody #2738

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Flow cytometric analysis of Ubiquitin conjugating enzyme E2 N expression in HT-1080 cells using anti-Ubiquitin conjugating enzyme E2 N antibody (Cat#2738, 1:2,000). Green, isotype control; red, Ubiquitin conjugating enzyme E2 N.

Immunocytochemical staining of HT-1080 cells with anti-Ubiquitin conjugating enzyme E2 N antibody(Cat#2738, 1:1,000) . Nuclei were stained blue with DAPI; Ubiquitin conjugating enzyme E2 N was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-ubiquitin conjugating enzyme E2 N antibody (Cat#2738). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ubiquitin conjugating enzyme E2 N antibody (Cat#2738, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

UBE2N
货号：

2738
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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Information

Product Name	Anti-UBE2N Rabbit Monoclonal Antibody
Aliases	UBE2N; Ubiquitin Conjugating Enzyme E2 N; UbcH-Ben; UBC13; Ubiquitin-Conjugating Enzyme E2N (Homologous To Yeast UBC13); Ubiquitin-Conjugating Enzyme E2N (UBC13 Homolog, Yeast); Ubiquitin-Conjugating Enzyme E2 N; E2 Ubiquitin-Conjugating Enzyme N; Ubiquitin Carrier Protein N; Ubiquitin-Protein Ligase N; MGC8489; UbcH13; Bendless-Like Ubiquitin Conjugating Enzyme; Bendless-Like Ubiquitin-Conjugating Enzyme; Epididymis Secretory Protein Li 71; Ubiquitin-Conjugating Enzyme E2N; Ubiquitin Conjugating Enzyme E2N; Yeast UBC13 Homolog; UBCHBEN; UBC13; EC 2.3.2.23; EC 6.3.2.19; HEL-S-71; UBCHBEN; Ubc13; BLU
Background	Gene Name: UBE2N NCBI Gene Entry: 7334 UniProt Entry: P61088
Application Information	Molecular Weight: Predicted, 17 kDa; observed, 17 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB2555 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Ube2N / Ubc13
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Ubiquitin conjugating enzyme E2 N expression in HT-1080 cells using anti-Ubiquitin conjugating enzyme E2 N antibody (Cat#2738, 1:2,000). Green, isotype control; red, Ubiquitin conjugating enzyme E2 N.
Immunocytochemical staining of HT-1080 cells with anti-Ubiquitin conjugating enzyme E2 N antibody(Cat#2738, 1:1,000) . Nuclei were stained blue with DAPI; Ubiquitin conjugating enzyme E2 N was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-ubiquitin conjugating enzyme E2 N antibody (Cat#2738). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ubiquitin conjugating enzyme E2 N antibody (Cat#2738, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

UBE2N
货号：

2738
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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